Toxic effect of C₆₀ fullerene-doxorubicin complex towards tumor and normal cells in vitro
Creation of new nanostructures possessing high antitumor activity is an important problem of modern biotechnology. Aim. To evaluate cytotoxicity of created complex of pristine C₆₀ fullerene with the anthracycline antibiotic doxorubicin (Dox), as well as of free C₆₀ fullerene and Dox, towards differe...
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| Datum: | 2014 |
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| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | English |
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Інститут молекулярної біології і генетики НАН України
2014
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| Schriftenreihe: | Вiopolymers and Cell |
| Schlagworte: | |
| Online Zugang: | https://nasplib.isofts.kiev.ua/handle/123456789/154553 |
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| Назва журналу: | Digital Library of Periodicals of National Academy of Sciences of Ukraine |
| Zitieren: | Toxic effect of C₆₀ fullerene-doxorubicin complex towards tumor and normal cells in vitro / S.V. Prylutska, G.V. Didenko, G.P. Potebnya, K.I. Bogutska, Yu.I. Prylutskyy, U. Ritter, P. Scharff // Вiopolymers and Cell. — 2014. — Т. 30, № 5. — С. 372-376. — Бібліогр.: 25 назв. — англ. |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine| Zusammenfassung: | Creation of new nanostructures possessing high antitumor activity is an important problem of modern biotechnology. Aim. To evaluate cytotoxicity of created complex of pristine C₆₀ fullerene with the anthracycline antibiotic doxorubicin (Dox), as well as of free C₆₀ fullerene and Dox, towards different cell types – tumor, normal immunocompetent and hepatocytes. Methods. Measurement of size distribution for particles in C₆₀ + Dox mixture was performed by a dynamic light scattering (DLS) technique. Toxic effect of C₆₀+ Dox complex in vitro towards tumor and normal cells was studied using the MTT assay. Results. DLS experiment demonstrated that the main fraction of the particles in C₆₀+ Dox mixture had a diameter in the range of about 132 nm. The toxic effect of C₆₀ + Dox complex towards normal (lymphocytes, macrophages, hepatocytes) and tumor (Ehrlich ascites carcinoma, leukemia L1210, Lewis lung carcinoma) cells was decreased by ~10–16 % and ~7–9 %, accordingly, compared with the same effect of free Dox. Conclusions. The created C₆₀ + Dox composite may be considered as a new pharmacological agent that kills effectively tumor cells in vitro and simultaneously prevents a toxic effect of the free form of Dox on normal cells. |
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