Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Me...
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Інститут молекулярної біології і генетики НАН України
2013
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nasplib_isofts_kiev_ua-123456789-1778222025-02-09T12:19:58Z Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши Immunofluorescent analysis of the structure of synaptonemal complex damage induced by xenobiotics in mice spermatocytes Ацаева, М.М. Коломиец, О.Л. Генетика людини та медична генетика Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the introduction of 1,1-DMH and CP tо male mice. The most common damage were fragmentation of SCs, the disturbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of meiosis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced by the detection of abnormal sperm. Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analysis. 2013 Article Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши / М.М. Ацаева, О.Л. Коломиец // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 284-286. — Бібліогр.: 11 назв. — рос. 2219-3782 https://nasplib.isofts.kiev.ua/handle/123456789/177822 ru Фактори експериментальної еволюції організмів application/pdf Інститут молекулярної біології і генетики НАН України |
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Digital Library of Periodicals of National Academy of Sciences of Ukraine |
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DSpace DC |
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Russian |
| topic |
Генетика людини та медична генетика Генетика людини та медична генетика |
| spellingShingle |
Генетика людини та медична генетика Генетика людини та медична генетика Ацаева, М.М. Коломиец, О.Л. Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши Фактори експериментальної еволюції організмів |
| description |
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH) – a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the introduction of 1,1-DMH and CP tо male mice. The most common damage were fragmentation of SCs, the disturbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of meiosis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced by the detection of abnormal sperm.
Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analysis. |
| format |
Article |
| author |
Ацаева, М.М. Коломиец, О.Л. |
| author_facet |
Ацаева, М.М. Коломиец, О.Л. |
| author_sort |
Ацаева, М.М. |
| title |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_short |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_full |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_fullStr |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_full_unstemmed |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| title_sort |
иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши |
| publisher |
Інститут молекулярної біології і генетики НАН України |
| publishDate |
2013 |
| topic_facet |
Генетика людини та медична генетика |
| url |
https://nasplib.isofts.kiev.ua/handle/123456789/177822 |
| citation_txt |
Иммунофлуоресцентный анализ нарушений в структуре синаптонемных комплексов, вызванных ксенобиотиками в сперматоцитах мыши / М.М. Ацаева, О.Л. Коломиец // Фактори експериментальної еволюції організмів: Зб. наук. пр. — 2013. — Т. 13. — С. 284-286. — Бібліогр.: 11 назв. — рос. |
| series |
Фактори експериментальної еволюції організмів |
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AT acaevamm immunofluorescentnyjanaliznarušenijvstrukturesinaptonemnyhkompleksovvyzvannyhksenobiotikamivspermatocitahmyši AT kolomiecol immunofluorescentnyjanaliznarušenijvstrukturesinaptonemnyhkompleksovvyzvannyhksenobiotikamivspermatocitahmyši AT acaevamm immunofluorescentanalysisofthestructureofsynaptonemalcomplexdamageinducedbyxenobioticsinmicespermatocytes AT kolomiecol immunofluorescentanalysisofthestructureofsynaptonemalcomplexdamageinducedbyxenobioticsinmicespermatocytes |
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2025-11-25T23:30:36Z |
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| fulltext |
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6. Allen J.W., Gibson J.B., Poorman P. ., Backer L.C., Moses M.J. Synaptonemal Complex Damage Induced by
Clustogenic and Anti-Mitotic Chemicals: Implications for Nondisjunc-tions and Aneuploidy. Mutat. Res. – 1988.–
Vol. 201. – P. 313–324.
7. Counce S.J., Meyer G.F. Differentiation of the synaptonemal complex and the kinetochore in Locusta spermato-
cytes studied by whole mount electron microscopy // Chromosoma. – 1973. –Vol. 44. – P. 231–253.
8. Homolka D., Ivanek R., Capkova J., Jansa P., Forejt J. Chromosomal rearrangement interferes with meiotic X
chromosome inactivation // Genome Res. – 2007. – Vol. 17, 10. – P. 1431–1437.
9. Moses M.J. Microspreading and synaptonemal complex in cytogenetic study // Chromosomes Today. –1977. – Vol
6. – P. 71–82.
10. Navarro J., Vidal R., Quitart M., Egozcue J. A method for the sequential study of synaptonemal complex by light
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11. Turner J.M.A., Mahadevaiah S.K., Ellis P.J.I., Mitchell M.J., Burgoyne P.S. Pachytene asynapsis drives meiotic
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ATSAEVA M.M., KOLOMIETS O.L.
N.I. Vavilov Institute of General Genetics, RAS
Russia, 119991, Moscow, Gubkin str., 3, e-mail: olkolomiets@mail.ru
IMMUNOFLUORESCENT ANALYSIS OF THE STRUCTURE OF SYNAPTONEMAL
COMPLEX DAMAGE INDUCED BY XENOBIOTICS IN MICE SPERMATOCYTES
Aims. The aim of this study was a comparative analysis of the effects of 1,1-dimethyl hydrazine (1,1-DMH)
– a component of rocket fuel, and anti-cancer drug cyclophosphan (CP) to the structure of the synaptonemal
complex (SC) of male mice and selection of the defective spermatocytes I at meiosis. Methods. Immuno-
fluorescent analysis of the structure of SC damage and chromatin silencing. Results. Multiple damage in the
structure of the SCs or even the absence of SC has been detected at the spermatocytes nuclei after the intro-
duction of 1,1-DMH and CP t male mice. The most common damage were fragmentation of SCs, the dis-
turbance of the chromosome synapsis and architectonics of the nuclei. Ring chromosome and SCP3 protein
aggregates were also found. There were every indication of pachytene arrest at many nuclei. We revealed
morphological features, the so-called meiotic catastrophe that blocked meiosis at the different stages of mei-
osis. Conclusions. 1,1-DMH and CP cause dramatic damage of meiotic chromosomes, which carries a risk
of disruption of spermatogenesis, infertility and risk of chromosomal aberrations of offspring, as evidenced
by the detection of abnormal sperm.
Key words: synaptonemal complex, spermatocytes, mice, 1,1-dimethyl hydrazine, immunofluorescent analy-
sis.
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