Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression

• Date: 2010
• Main Authors: Sindarovska, Y.R., Gerasymenko, I.M., Sheludko, Y.V., Olevinskaya, Z.M., Spivak, N.Y., Kuchuk, N.V.
• Format: Article
• Language: English
• Published: Інститут клітинної біології та генетичної інженерії НАН України 2010
• Series: Цитология и генетика
• Subjects: Оригинальные работы
• Online Access: https://nasplib.isofts.kiev.ua/handle/123456789/66797
• Journal Title: Digital Library of Periodicals of National Academy of Sciences of Ukraine
• Cite this: Production of human interferon alfa 2b in plants of Nicotiana excelsior by Agrobacterium-mediated transient expression / Y.R. Sindarovska, I.M. Gerasymenko, Y.V. Sheludko, Z.M. Olevinskaya, N.Y. Spivak, N.V. Kuchuk // Цитология и генетика. — 2010. — Т. 44, № 5. — С. 60-64. — Бібліогр.: 32 назв. — англ.

Human interferon α2b gene was transiently expressed in Nicotiana excelsior plants. Fusion with N. plumbaginifolia calreticulin signal peptide for improved apoplast targeting and carrying out the expression under optimized conditions resulted in maximal interferon activity of 3.2 • 10³ IU/g fresh wei...