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Метрологічна характеристика розробленої методики визначення антиоксидантної активності аскорбінової кислоти

Метрологічна характеристика розробленої методики визначення антиоксидантної активності аскорбінової кислоти

The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2...

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Bibliographic Details
Main Authors: Maslov, Oleksandr Yu., Kolisnyk, Sergii V., Komisarenko, Mykola A., Akhmedov, Elshan Yu., Koval, Alla O., Kostina, Tatiana A., Kolisnyk, Olena V.
Format: Article
Language:English
Published: National University of Pharmacy 2023
Subjects:
антиоксидантна активність
потенціометричний метод
валідація
аскорбінова кислота
antioxidant activity
potentiometric method
validation
ascorbic acid
Online Access:https://ophcj.nuph.edu.ua/article/view/276394
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Summary:The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2 = 0.9993). The percentage of recovery was found to be in the range from 95.38 to 105.00 %. The values of %RSD for repeatability and intermediate precision were 1.86 and 1.95 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %. It has been proven that the method developed is express, rapid, highly sensitive, accurate and sufficiently reliable.

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