Метрологічна характеристика розробленої методики визначення антиоксидантної активності аскорбінової кислоти

The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2...

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Datum:2023
Hauptverfasser: Maslov, Oleksandr Yu., Kolisnyk, Sergii V., Komisarenko, Mykola A., Akhmedov, Elshan Yu., Koval, Alla O., Kostina, Tatiana A., Kolisnyk, Olena V.
Format: Artikel
Sprache:English
Veröffentlicht: National University of Pharmacy 2023
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Online Zugang:https://ophcj.nuph.edu.ua/article/view/276394
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Назва журналу:Journal of Organic and Pharmaceutical Chemistry

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Journal of Organic and Pharmaceutical Chemistry
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Zusammenfassung:The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2 = 0.9993). The percentage of recovery was found to be in the range from 95.38 to 105.00 %. The values of %RSD for repeatability and intermediate precision were 1.86 and 1.95 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %. It has been proven that the method developed is express, rapid, highly sensitive, accurate and sufficiently reliable.