Изучение жизнеспособности микроорганизмов и сохранности структурных участков их ДНК, амплифицируемых в ПЦР, после низкотемпературного хранения в различных условиях

Изучение жизнеспособности микроорганизмов и сохранности структурных участков их ДНК, амплифицируемых в ПЦР, после низкотемпературного хранения в различных условиях

The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions...

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Bibliographic Details
Date:2003
Main Authors: Vysekantsev, I. P., Omelchenko, E. A., Gurina, T. M.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2003
Subjects:
мікроорганізми
кріоконсервування
ДНК
ПЦР
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1013
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions of storage temperature variation within the mentioned range, there are preserved the DNA structural sites, providing the amplification in polymerase chain reaction (PCR) of specific fragments.

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