Изучение жизнеспособности микроорганизмов и сохранности структурных участков их ДНК, амплифицируемых в ПЦР, после низкотемпературного хранения в различных условиях

The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Datum:2003
Hauptverfasser: Vysekantsev, I. P., Omelchenko, E. A., Gurina, T. M.
Format: Artikel
Sprache:English
Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2003
Schlagworte:
Online Zugang:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1013
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Назва журналу:Problems of Cryobiology and Cryomedicine

Institution

Problems of Cryobiology and Cryomedicine
Beschreibung
Zusammenfassung:The obtained experimental data testify to the fact that under low temperature preservation the cyclic changes in storage temperature from –196 to –100°C can result in additional death of microbial cells. In microbial cells, stored under liquid nitrogen constant temperature and under conditions of storage temperature variation within the mentioned range, there are preserved the DNA structural sites, providing the amplification in polymerase chain reaction (PCR) of specific fragments.