ЭПР-исследование окислительно-восстановительных реакций водно-солевых экстрактов плаценты человека: влияние температуры хранения и времени экстрагирования

• Datum: 2005
• Hauptverfasser: Repina, S. V., Nardid, O. A., Rozanova, E. D., Tsymbal, L. V., Schetinsky, M. I., Naumenko, E. I.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2005
• Schlagworte: екстракти плаценти людини; ЕПРспінових зондів; заморожування; білок-нуклеотидний склад; окислювально-відновні реакції
• Online Zugang: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1207
• Назва журналу: Problems of Cryobiology and Cryomedicine

Using EPR method there were investigated redox reactions of stable TEMPON nitroxyl radical with human placenta aqueoussaline extracts depending on placenta tissue storage temperature and extraction time. It was demonstrated, that with an increase in extraction time from 2 to 12 hrs, as well as placenta tissue storage at –20°C for a month, there was a decrease in redox process activity in extracts. Freezing of placenta tissue down to –196°C did not significantly affect redox activity of extracts in comparison with those from native placenta. Investigation results of protein-nucleotide composition in the obtained aqueous-saline extracts testify to the fact, that high-molecular extract fragments possess higher reductive activity in respect of TEMPON.