Особенности белково-пептидного и углеводного состава супернатантов из личинок Tenebrio molitor

• Date: 2016
• Main Authors: Gulevsky, Aleksandr K., Tretiak, Diana V.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Subjects: Tenebrio molitor; холодова аклімація; хроматографія; пептиди; цукри; цукроспирти
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1241
• Journal Title: Problems of Cryobiology and Cryomedicine

The paper describes the chromatographic studies on the molecular-mass distribution of proteins and peptides of supernatants, derived from Tenebrio molitor larvae during cold acclimation. Supernatants of non-acclimated larvae of T. molitor had the highest amount of peptide fractions. It has been shown that cold-acclimated T. molitor larvae comprised the low-molecular peptide fractions with MW of (540 ± 20) – (2.255 ± 85) Da, and high-molecular peptides with MW of (4.675 ± 225) – (6.595 ± 550) Da were characteristic for non-acclimated larvae. The content of sugars and polyols in supernatants of cold-acclimated and non-acclimated T. molitor larvae was determined. It has been found that glucose concentration in non-acclimated T. molitor larvae was in 1.34 times higher than in cold-acclimated ones. Аcclimated T. molitor larvae had more hydrophilic and less hydrophobic proteins as compared to non-acclimated ones. As well it has been established that non-acclimated T. molitor larvae had low concentrations of polyols (sorbitol and mannite), which were absent in acclimated insects.Probl Cryobiol Cryomed 2016; 26(4): 322–330