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Криозащитная эффективность ряда криопротекторов в зависимости от скорости охлаждения

Криозащитная эффективность ряда криопротекторов в зависимости от скорости охлаждения

The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell surv...

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Bibliographic Details
Main Authors: Chernobai, N. A., Gurina, T. M., Pakhomov, A. V.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2024
Subjects:
кріоконсервування
швидкість охолодження
клітини інтерстиція тестисів
проникність
кріопротектори
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/125
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Summary:The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell survival under all the studied cooling rates. Cell survival increased (from 56% upto 83%) if cooling rates were elevated from 1 up to 10 deg/min in the case of other cryoprotectants. Cooling rates about 10 deg/min provided sufficiently high level of post-thaw cell survival.

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