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The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell surv...
Збережено в:
Дата: | 2024 |
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Автори: | , , |
Формат: | Стаття |
Мова: | English |
Опубліковано: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2024
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Теми: | |
Онлайн доступ: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/125 |
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Назва журналу: | Problems of Cryobiology and Cryomedicine |
Репозитарії
Problems of Cryobiology and CryomedicineРезюме: | The survival of testes interstitial cells after freeze-thawing using the cooling rates of 1; 5 and 10 deg/min and glycerol (7%), dimethyl sulfoxide (10%), ethylene glycol (7%) and dimethyl formamide (5%) solutions has been estimated. Using of glycerol provided sufficiently high (up to 75%) cell survival under all the studied cooling rates. Cell survival increased (from 56% upto 83%) if cooling rates were elevated from 1 up to 10 deg/min in the case of other cryoprotectants. Cooling rates about 10 deg/min provided sufficiently high level of post-thaw cell survival. |
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