Зависимость проницаемости мембран клеток интерстиция тестисов для молекул ряда криопротекторов от температуры

• Datum: 2010
• Hauptverfasser: Chernobai, N. A., Pakhomov, A. V., Kovalenko, I. F., Bozhok, G. A., Kovalenko, S. Ye., Rozanov, L. F.
• Format: Artikel
• Sprache: English
• Veröffentlicht: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2010
• Schlagworte: коефіцієнти проникності; енергія активації; клітини інтерстицію тестисів; волюмометрія; кріопротектори
• Online Zugang: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/204
• Назва журналу: Problems of Cryobiology and Cryomedicine

Osmotic behavior of the rat testis intersticium cells in cryoprotectants solutions – substances belonging to alcohols (ethylene glycol (EG), glycerol and 1,2-butane diol (1,2-BD)), oxides (dimethyl sulfoxide (Me2SO)) and amides (dimethyl formamide (DMFA)) was studied. Permeability coefficients of testis cells for these cryoprotectants were determined at 35, 20 and 5°C by volumetric analysis and the Kedem-Kachalsky modified physico-mathematical model. The calculated values of the activation energies for EG, glycerol, 1,2-BD, Me2SO and DMFA molecules are 53.28, 46.92, 36.33, 58.89, 62.99 kJ/mol, respectively. Testis cell membranes were established to be equally well permeable for EG, glycerol, 1,2-BD and Me2SO, and their permeability for DMFA at 35°C was comparable with that for water molecules.