УÑÐ»Ð¾Ð²Ð¸Ñ Ð²Ð¸Ñ‚Ñ€Ð¸Ñ„Ð¸ÐºÐ°Ñ†Ð¸Ð¸ Ñмбрионов вьюна (Misgurnus fossilis) в криозащитных Ñредах
Resistance of loach (Misgurnus fossilis) embryos to the effect of 5 cryoprotective media was investigated and the conditions of their vitrification were determined. The obtained experimental data demonstrated, that at a developmental stage, corresponding to the heart beating onset, the highest numbe...
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| Date: | 2009 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Published: |
Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
2009
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| Subjects: | |
| Online Access: | https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/270 |
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| Journal Title: | Problems of Cryobiology and Cryomedicine |
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Problems of Cryobiology and Cryomedicine| Summary: | Resistance of loach (Misgurnus fossilis) embryos to the effect of 5 cryoprotective media was investigated and the conditions of their vitrification were determined. The obtained experimental data demonstrated, that at a developmental stage, corresponding to the heart beating onset, the highest number of vitrified embryos (up to 100%) was observed during 30 min incubation within a cryoprotective medium, consisting of 30% sucrose, 10% ethylene glycol, 3% polyethylene oxide–1500 and 20% 1,2-propane diol. Vitrification temperature of loach embryos at this developmental stage was within –80...–176°C range. |
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