Разработка способа криоконсервирования вируса болезни Марека

• Date: 2013
• Main Authors: Stegniy, M. Yu., Stegniy, B. T., Goltsev, A. N.
• Format: Article
• Language: Russian
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Subjects: криоконсервування; вірус; хвороба Марека; вакцина
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/342
• Journal Title: Problems of Cryobiology and Cryomedicine

The cryopreservation method, consisting in application of two-stage freezing regimen down to -30°C with 1°C/min rate, from –30 down to –70°C with 5–10°C/min rate with following plunging into liquid nitrogen (–196°C) at that composition of protective media 199 nutrient media 80% with add...