Влияние скорости охлаждения в различных температурных интервалах на сохранность стероидогенного потенциала суспензии интерстициальных клеток семенников взрослых крыс

Влияние скорости охлаждения в различных температурных интервалах на сохранность стероидогенного потенциала суспензии интерстициальных клеток семенников взрослых крыс

The effect of cryopreservation conditions (cryoprotectant concentration, cell incubation with cryoprotectants prior to freezing, cooling of samples with various temperature intervals) on survival of steroidogenic cells in the testscular interstitium cell suspension (ICS) has been investigated. This...

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Bibliographic Details
Date:2007
Main Authors: Gurina, T. M., Pakhomov, A. V., Bozhok, G. A.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
Subjects:
тестостерон
клітини Лейдига
швидкість охолодження
3β-гідроксистероїддегідрогеназа
переохолодження
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/486
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:The effect of cryopreservation conditions (cryoprotectant concentration, cell incubation with cryoprotectants prior to freezing, cooling of samples with various temperature intervals) on survival of steroidogenic cells in the testscular interstitium cell suspension (ICS) has been investigated. This resulted in designing of cryopreservation protocol for ICS, which provided the highest survival and functional activity of steroidogenic cells if compared with the protocols already existing for testicular biological material.

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