Применение метода пластической деформации для определения пороговых концентраций криопротекторных веществ при криоконсервировании дрожжевых клеток Saccharomyces cerevisiae

Применение метода пластической деформации для определения пороговых концентраций криопротекторных веществ при криоконсервировании дрожжевых клеток Saccharomyces cerevisiae

Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion t...

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Bibliographic Details
Date:2007
Main Authors: Osetsky, A. I., Kirilyuk, A. L., Gurina, T. M., Vysekantsev, I. P.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
Subjects:
пластична деформація
межа текучості
криві напруга-деформація
гліцерин
ДМСО
ПЕО-1500
Saccharomyces cerevisiae
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/534
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:Plastic strain of glycerol and PEO-1500 frozen aqueous solutions has been studied and an alteration in their plastic characteristics when achieving certain (threshold) concentrations of cryoprotective substances has been established. This change is associated to local closed liquid phase inclusion transition into the through liquid interlayers, that excludes an internal pressure development and corresponding mechanical damages in biological objects being under cryopreservation. This conclusion is confirmed by the experiments on Saccharomyces cerevisiae yeast cell cryopreservation.

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