Изучение влияния холодового закаливания картофеля на сохранность меристем, криоконсервированных медленным замораживанием

• Дата: 2006
• Автори: Stribul, T. F., Shevchenko, N. A., Rozanov, L. F.
• Формат: Стаття
• Мова: English
• Опубліковано: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2006
• Теми: meristem (apex); cold hardening; cryoprotectants; cryopreservation; freezing; integrity; меристема (апекс); холодове загартовування; кріопротектори; кріоконсервування; заморожування; збереженість; холодовая закалка; криопротекторы; криоконсервирование; замораживание; сохранность
• Онлайн доступ: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/617
• Назва журналу: Problems of Cryobiology and Cryomedicine

Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in comparison with the same index in non-hardened meristems. Potato apex hardening is inexpedient when using 1,2-PD. Observation for cryopreserved meristem development within 30 days has demonstrated that treatment with cryoprotectants and freezing inhibit an apex growth in comparison with the control. 1,2-PD occurred to be less toxic. DMSO treatment can cause callus-genesis.