Изучение влияния холодового закаливания картофеля на сохранность меристем, криоконсервированных медленным замораживанием

Изучение влияния холодового закаливания картофеля на сохранность меристем, криоконсервированных медленным замораживанием

Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in com...

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Bibliographic Details
Date:2006
Main Authors: Stribul, T. F., Shevchenko, N. A., Rozanov, L. F.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2006
Subjects:
меристема (апекс)
холодове загартовування
кріопротектори
кріоконсервування
заморожування
збереженість
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/617
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Journal Title:Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine
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Summary:Cold hardening effect on potato meristem integrity at two-step cryopreservation has been studied. Such cryoprotectants as dimethyl sulfoxide (DMSO) and 1,2-propanediol (1,2-PD) have been used. Freezing of hardened potato apexes under DMSO protection was established to increase their integrity in comparison with the same index in non-hardened meristems. Potato apex hardening is inexpedient when using 1,2-PD. Observation for cryopreserved meristem development within 30 days has demonstrated that treatment with cryoprotectants and freezing inhibit an apex growth in comparison with the control. 1,2-PD occurred to be less toxic. DMSO treatment can cause callus-genesis.

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