The permeability coefficients for one- and two-cell embryos for ethylene glycol (EG) and water molecules using the volumetry method and physical-chemical model, describing the mass exchange processes between a cell and its environment, were determined. The cleavage was shown as not affecting the tra...

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Datum:	2004
1. Verfasser:	Pishko, O. V.
Format:	Artikel
Sprache:	English
Veröffentlicht:	Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2004
Schlagworte:	ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½ ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ–
Online Zugang:	https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/689
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Назва журналу:	Problems of Cryobiology and Cryomedicine

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Problems of Cryobiology and Cryomedicine

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spelling	oai:ojs.pkp.sfu.ca:article-6892025-12-02T15:25:02Z Effect of Ethylene Glycol Concentration on Membrane Permeability of One- and Two Cell Murine Embryos Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸ Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸ Pishko, O. V. ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÑÑ„Ñ„Ð¸Ñ†Ð¸ÐµÐ½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚Ð¸ embryo ethylene glycol permeability coefficient ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½ ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ– The permeability coefficients for one- and two-cell embryos for ethylene glycol (EG) and water molecules using the volumetry method and physical-chemical model, describing the mass exchange processes between a cell and its environment, were determined. The cleavage was shown as not affecting the transport characteristics of membranes, but augmenting their resistance to the increased cryoprotectant concentration effect. ÐžÐ¿Ñ€ÐµÐ´ÐµÐ»ÐµÐ½Ñ‹ ÐºÐ¾ÑÑ„Ñ„Ð¸Ñ†Ð¸ÐµÐ½Ñ‚Ñ‹ Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚Ð¸ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð´Ð»Ñ Ð¼Ð¾Ð»ÐµÐºÑƒÐ» ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ (ÐÐ“) Ð¸ Ð²Ð¾Ð´Ñ‹ Ñ Ð¸ÑÐ¿Ð¾Ð»ÑŒÐ·Ð¾Ð²Ð°Ð½Ð¸ÐµÐ¼ Ð¼ÐµÑ‚Ð¾Ð´Ð° Ð²Ð¾Ð»ÑŽÐ¼Ð¾Ð¼ÐµÑ‚Ñ€Ð¸Ð¸ Ð¸ Ñ„Ð¸Ð·Ð¸ÐºÐ¾-Ð¼Ð°Ñ‚ÐµÐ¼Ð°Ñ‚Ð¸Ñ‡ÐµÑÐºÐ¾Ð¹ Ð¼Ð¾Ð´ÐµÐ»Ð¸, Ð¾Ð¿Ð¸ÑÑ‹Ð²Ð°ÑŽÑ‰ÐµÐ¹ Ð¿Ñ€Ð¾Ñ†ÐµÑÑÑ‹ Ð¼Ð°ÑÑÐ¾Ð¾Ð±Ð¼ÐµÐ½Ð° Ð¼ÐµÐ¶Ð´Ñƒ ÐºÐ»ÐµÑ‚ÐºÐ¾Ð¹ Ð¸ Ð¾ÐºÑ€ÑƒÐ¶Ð°ÑŽÑ‰ÐµÐ¹ ÐµÐµ ÑÑ€ÐµÐ´Ð¾Ð¹. ÐŸÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, Ñ‡Ñ‚Ð¾ Ð´Ñ€Ð¾Ð±Ð»ÐµÐ½Ð¸Ðµ Ð½Ðµ Ð¾Ñ‚Ñ€Ð°Ð¶Ð°ÐµÑ‚ÑÑ Ð½Ð° Ñ‚Ñ€Ð°Ð½ÑÐ¿Ð¾Ñ€Ñ‚Ð½Ñ‹Ñ… Ñ…Ð°Ñ€Ð°ÐºÑ‚ÐµÑ€Ð¸ÑÑ‚Ð¸ÐºÐ°Ñ… Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½, Ð½Ð¾ Ð¿Ð¾Ð²Ñ‹ÑˆÐ°ÐµÑ‚ Ð¸Ñ… ÑƒÑÑ‚Ð¾Ð¹Ñ‡Ð¸Ð²Ð¾ÑÑ‚ÑŒ Ðº Ð´ÐµÐ¹ÑÑ‚Ð²Ð¸ÑŽ ÑƒÐ²ÐµÐ»Ð¸Ñ‡ÐµÐ½Ð½Ñ‹Ñ… ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¹ ÐºÑ€Ð¸Ð¾Ð¿Ñ€Ð¾Ñ‚ÐµÐºÑ‚Ð¾Ñ€Ð°. Ð’Ð¸Ð·Ð½Ð°Ñ‡ÐµÐ½Ð¾ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚Ð¸ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ– Ð¾Ð´Ð½Ð¾- Ñ‚Ð° Ð´Ð²Ð¾ÐºÐ»Ñ–Ñ‚Ð¸Ð½Ð½Ð¸Ñ… ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½Ñ–Ð² Ð¼Ð¸ÑˆÑ– Ð´Ð»Ñ Ð¼Ð¾Ð»ÐµÐºÑƒÐ» ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŽ Ñ‚Ð° Ð²Ð¾Ð´Ð¸ Ð· Ð²Ð¸ÐºÐ¾Ñ€Ð¸ÑÑ‚Ð°Ð½Ð½ÑÐ¼ Ð¼ÐµÑ‚Ð¾Ð´Ñƒ Ð²Ð¾Ð»ÑŽÐ¼Ð¾Ð¼ÐµÑ‚Ñ€Ñ–Ñ— Ñ– Ñ„Ñ–Ð·Ð¸ÐºÐ¾-Ð¼Ð°Ñ‚ÐµÐ¼Ð°Ñ‚Ð¸Ñ‡Ð½Ð¾Ñ— Ð¼Ð¾Ð´ÐµÐ»Ñ–, ÑÐºÐ° Ð¾Ð¿Ð¸ÑÑƒÑ” Ð¿Ñ€Ð¾Ñ†ÐµÑÐ¸ Ð¼Ð°ÑÐ¾Ð¾Ð±Ð¼Ñ–Ð½Ñƒ Ð¼Ñ–Ð¶ ÐºÐ»Ñ–Ñ‚Ð¸Ð½Ð¾ÑŽ Ñ‚Ð° Ð¾Ñ‚Ð¾Ñ‡ÑƒÑŽÑ‡Ð¸Ð¼ Ñ—Ñ— ÑÐµÑ€ÐµÐ´Ð¾Ð²Ð¸Ñ‰ÐµÐ¼. ÐŸÐ¾ÐºÐ°Ð·Ð°Ð½Ð¾, Ñ‰Ð¾ Ð´Ñ€Ð¾Ð±Ð»ÐµÐ½Ð½Ñ Ð½Ðµ Ð²Ð¿Ð»Ð¸Ð²Ð°Ñ” Ð½Ð° Ñ‚Ñ€Ð°Ð½ÑÐ¿Ð¾Ñ€Ñ‚Ð½Ñ– Ñ…Ð°Ñ€Ð°ÐºÑ‚ÐµÑ€Ð¸ÑÑ‚Ð¸ÐºÐ¸ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½, Ð°Ð»Ðµ Ð¿Ñ–Ð´Ð²Ð¸Ñ‰ÑƒÑ” Ñ—Ñ… ÑÑ‚Ñ–Ð¹ÐºÑ–ÑÑ‚ÑŒ Ð´Ð¾ Ð´Ñ–Ñ— Ð·Ð±Ñ–Ð»ÑŒÑˆÐµÐ½Ð¸Ñ… ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ñ–Ð¹ ÐºÑ€Ñ–Ð¾Ð¿Ñ€Ð¾Ñ‚ÐµÐºÑ‚Ð¾Ñ€Ð°. Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2004-06-15 Article Article Research article application/pdf https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/689 Problems of Cryobiology and Cryomedicine; No. 2 (2004): Problems of Cryobiology; 56-61 Проблемы криобиологии и криомедицины; № 2 (2004): Problems of Cryobiology; 56-61 Проблеми кріобіології і кріомедицини; № 2 (2004): Problems of Cryobiology; 56-61 2518-7376 2307-6143 en https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/689/722 Copyright (c) 2020 O. V. Pishko https://creativecommons.org/licenses/by/4.0
institution	Problems of Cryobiology and Cryomedicine
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datestamp_date	2025-12-02T15:25:02Z
collection	OJS
language	English
topic	ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½ ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ–
spellingShingle	ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½ ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ– Pishko, O. V. Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
topic_facet	ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÑÑ„Ñ„Ð¸Ñ†Ð¸ÐµÐ½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚Ð¸ embryo ethylene glycol permeability coefficient ÐµÐ¼Ð±Ñ€Ñ–Ð¾Ð½ ÐµÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ñ–ÐºÐ¾Ð»ÑŒ ÐºÐ¾ÐµÑ„Ñ–Ñ†Ñ–Ñ”Ð½Ñ‚ Ð¿Ñ€Ð¾Ð½Ð¸ÐºÐ½Ð¾ÑÑ‚Ñ–
format	Article
author	Pishko, O. V.
author_facet	Pishko, O. V.
author_sort	Pishko, O. V.
title	Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
title_short	Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
title_full	Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
title_fullStr	Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
title_full_unstemmed	Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
title_sort	ð’ð»ð¸ñð½ð¸ðµ ðºð¾ð½ñ†ðµð½ñ‚ñ€ð°ñ†ð¸ð¸ ññ‚ð¸ð»ðµð½ð³ð»ð¸ðºð¾ð»ñ ð½ð° ð¿ñ€ð¾ð½ð¸ñ†ð°ðµð¼ð¾ññ‚ñœ ð¼ðµð¼ð±ñ€ð°ð½ ð¾ð´ð½ð¾- ð¸ ð´ð²ñƒñ…ðºð»ðµñ‚ð¾ñ‡ð½ñ‹ñ… ñð¼ð±ñ€ð¸ð¾ð½ð¾ð² ð¼ñ‹ñˆð¸
title_alt	Effect of Ethylene Glycol Concentration on Membrane Permeability of One- and Two Cell Murine Embryos Ð’Ð»Ð¸ÑÐ½Ð¸Ðµ ÐºÐ¾Ð½Ñ†ÐµÐ½Ñ‚Ñ€Ð°Ñ†Ð¸Ð¸ ÑÑ‚Ð¸Ð»ÐµÐ½Ð³Ð»Ð¸ÐºÐ¾Ð»Ñ Ð½Ð° Ð¿Ñ€Ð¾Ð½Ð¸Ñ†Ð°ÐµÐ¼Ð¾ÑÑ‚ÑŒ Ð¼ÐµÐ¼Ð±Ñ€Ð°Ð½ Ð¾Ð´Ð½Ð¾- Ð¸ Ð´Ð²ÑƒÑ…ÐºÐ»ÐµÑ‚Ð¾Ñ‡Ð½Ñ‹Ñ… ÑÐ¼Ð±Ñ€Ð¸Ð¾Ð½Ð¾Ð² Ð¼Ñ‹ÑˆÐ¸
description	The permeability coefficients for one- and two-cell embryos for ethylene glycol (EG) and water molecules using the volumetry method and physical-chemical model, describing the mass exchange processes between a cell and its environment, were determined. The cleavage was shown as not affecting the transport characteristics of membranes, but augmenting their resistance to the increased cryoprotectant concentration effect.
publisher	Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine
publishDate	2004
url	https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/689
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first_indexed	2025-07-22T19:35:03Z
last_indexed	2025-12-02T15:46:49Z
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