Влияние криозащитных растворов и их компонентов на интенсивность процессов перекисного окисления липидов и антиоксидантную активность лейкоцитов при криоконсервировании

• Date: 2013
• Main Authors: Khudyakov, Andrey N., Polezhaeva, Tatyana V., Zaitseva, Oksana O., Laptev, Denis S., Solomina, Olga N., Kostyaev, Andrey A.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2013
• Subjects: лейкоцити; кріопротектори; кріоконсерванти; перекисне окислення ліпідів; антиоксидантна активність
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/9
• Journal Title: Problems of Cryobiology and Cryomedicine

Using chemiluminescence method it was shown that after addition of several cryoprotectants 1,2-propane diol (21% initial concentration hereinafter and added 1:1) and dimethyl sulfoxide (8%) to leukocyte suspension the level of LPO and antioxidant activity decreased. Presence of glycerol (7%) and hexa-methylene bis-tetra hydroxyethyl urea (30%) resulted in simultaneous stimulation of LPO and antioxidant systems. Hydroxyethylated starch (5.7%) and gelatine (molecular weight of 16,000; 7.5%) increase LPO. After storage in combined cryoprotective media at –10, –20 and –40°C the leukocytes revealed the activation of antioxidant systems on the background of stable LPO indices, that, probably, contributed to morphofunctional preservation of 75% of cells. Probl Cryobiol Cryomed 2013; 23(1):49–57.