Влияние инкапсуляции в альгинатные микросферы на жизнеспособность мезенхимальных стромальных клеток после экспозиции с проникающими криопротекторами

• Date: 2016
• Main Authors: Zaikov, Vedeney S., Tarusin, Dmitriy N., Petrenko, Aleksandr Yu.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Subjects: мезенхімальні стромальні клітини; альгінатні мікросфери; вітрифікація; мультикомпонентний розчин кріопротекторів; диметилсульфоксид; етиленгліколь; 1; 2-пропандіол
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/938
• Journal Title: Problems of Cryobiology and Cryomedicine

The effect of encapsulation into alginate microspheres (AMS) on viability of mesenchymal stromal cells (MSCs) after exposure to the solutions of penetrating cryoprotectants dimethyl sulfoxide (DMSO), ethylene glycol (EG) and 1,2-propane diol (1,2‑PD) with concentrations varying from 1.5 to 9 M during 1.5 and 5 min was studied. It was shown that the viability of MSCs both as a suspension and encapsulated in AMS decreased with a rise in cryoprotectant concentration and exposure time. Encapsulation into AMS significantly protected the cells from cytotoxic damage of the cryoprotectants, e. g. if the 5-min exposure to 9 M DMSO, EG and 1,2-PD resulted in the death of quite all the cells in the suspension, the viability of encapsulated MSCs after exposure under similar conditions made 60, 80 and 52% respectively. It was found that the studied penetrating cryoprotectants had a various cytotoxicity to MSCs, and it  decreased in the row DMSO > 1,2-PD > EG. The findings can be used to develop a multi-component vitrifying solution. Probl Cryobiol Cryomed 2016; 26(3): 213–220