Морфологические особенности ткани селезенки после введения фторурацила и криоконсервированных ядросодержащих клеток кордовой крови

• Date: 2016
• Main Authors: Kudokotseva, Olga V., Volina, Viktoriya V., Lomakin, Ivan I.
• Format: Article
• Language: English
• Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2016
• Subjects: селезінка; імуно- та гемосупресія; фторурацил; кріоконсервовані клітини кордової крові
• Online Access: https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/942
• Journal Title: Problems of Cryobiology and Cryomedicine

We studied the effect of administered cryopreserved nucleated cells of human cord blood (cCBNCs) on murine spleen tissue morphology under hemo- and immunosuppression induced by a single intraperitoneal injection of 5-fluorouracil (5-FU) antimetabolite of nucleic metabolism in the maximum tolerated dose. Progressing changes, typical for a damage caused by toxic substances, such as: congestion, exudation and leukocytes infiltration of spleen pulp; accumulation of macrophages either with phagocytized lymphocytes or their fragments; degenerative and necrotic changes in tissue elements of the pulp and follicles were revealed in spleen following  5‑FU introduction within entire observation period (16 days). A high degree of murine spleen reactivity in response to the administration of cryopreserved cord blood cells was found. To day 2 after cCBNCs administration we observed structural rearrangements in spleen, and already 10 days later 5-FU antimetabolite toxic injury the organ structure was back to the norm. Probl Cryobiol Cryomed 2016; 26(3): 249–258