ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ
The strain of Rhizobium galegae К- 3 - active symbiote of Galega orientalis has been received by means of selection. Positive influence of this strain on formation and functioning of symbiosis during the first and second years of cultivation of Galega orientalis plants is shown. R. galegae К- 3 prom...
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| Sprache: | Englisch Ukrainisch |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2016
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Agriciltural microbiology| _version_ | 1871465334302572544 |
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| author | Воробей, Ю. О. Воробей, В. С. Пиріг, О. В. |
| author_facet | Воробей, Ю. О. Воробей, В. С. Пиріг, О. В. |
| author_institution_txt_mv | [
{
"author": "Ю. О. Воробей",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "В. С. Воробей",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. В. Пиріг",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Воробей, Ю. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:42Z |
| description | The strain of Rhizobium galegae К- 3 - active symbiote of Galega orientalis has been received by means of selection. Positive influence of this strain on formation and functioning of symbiosis during the first and second years of cultivation of Galega orientalis plants is shown. R. galegae К- 3 promotes to formation of generous amount of indeterminate nodules on the roots, increase of activity of symbiotic nitrogen fixation and increase in the yield of Galega orientalis. |
| doi_str_mv | 10.35868/1997-3004.24.9-17 |
| first_indexed | 2025-07-17T12:23:04Z |
| format | Article |
| fulltext |
THE FEATURES OF INTERACTION RHIZOBIUM GALEGAE WITH GALEGA
ORIENTALIS
Yu. O. Vorobei, V. S. Vorobei, А. V. Piroh
Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS,
City of Chernihiv
The strain of Rhizobium galegae К- 3 - active symbiote of Galega orientalis has been
received by means of selection. Positive influence of this strain on formation and functioning of
symbiosis during the first and second years of cultivation of Galega orientalis plants is shown. R.
galegae К- 3 promotes to formation of generous amount of indeterminate nodules on the roots,
increase of activity of symbiotic nitrogen fixation and increase in the yield of Galega orientalis.
Key words: Rhizobium galegae, Galega orientalis, legume-rhizobial symbiosis
Today, significant attention is paid to
the introduction of different legumes with
economically valuable signs into the culture.
According to the literature, new macro-
cultural species, which are similar to their
wild relatives by physiological properties,
have a high symbiotic potential [1]. Use of
Galega orientalis in agriculture is due to its
high winter resistance and productivity,
intensive growth and longevity, early spring
regrowth of tops, the ability for utilization at
the same place for 10-12 years, as well as
high quality of feed obtained without the use
of nitrogen fertilizers – due to the symbiotic
nitrogen fixation [2; 3]. Increased ability of
Galega orientalis to fix atmospheric
nitrogen, prevalence of symbiotrophic
nutrition over autotrophic, high sensitivity to
inoculation with specific strains of rhizobia
have a positive impact of bacterization on
productivity of culture and quality of raw
materials.
Cultivation of Galega orientalis on
soils where no active strains of specific
rhizobia are present requires the use of
biopreparation for pre-seeding bacterization,
which in turn stipulates extensive research
of features of legume-rhizobial symbiosis
formation.
The aim of this paper was to study
features of relationships of Rhizobium
galegae with Galega orientalis plants.
Materials and methods. Study of the
inoculation efficiency of Galega orientalis
(variety Kavkazskyi branets) by the strains
of R. galegae was conducted under the
conditions of field experiment on sod-
podzolic sandy loam soil (pH – 6.47; humus
content – 0.8-1.1 %; easily-hydrolysed
nitrogen (by Tiuryn and Kononova) – 56.0-
57.0 mg; P2O5 – 160.0-170.0 mg and K2O
(by Kirsanov) – 100-110 mg per 1 kg of
soil). Planting was carried out by the wide-
row method, row spacing – 40 cm. Seeding
norm – 10 kg/ha. Before seeding, seeds were
scarified and inoculated with the strains of
rhizobia at a rate of 200-300 thous.
cells/seed. Standard strain of R. galegae
0703 obtained from the collection of
beneficial soil microorganisms [4] and strain
R. galegae K-3, isolated from nodules of
Galega orientalis were used for
bacterization.
Nodules were sampled from the roots
of Galega orientalis for cytological tests,
wash them out from the soil residues and
fixed by FAA (16 % formalin – 1 portion,
acetic acid (concentrated) – 1 portion; 96 %
alcohol – 5 portions). Glutaric aldehyde was
also used for fixing nodules. Fixative was
washed out in 80 % alcohol solution. Fixed
material was dehydrated by four-fold
washing in alcohol (from 96 % to 100 %),
keep in the solution containing of alcohol
and toluene with gradual alcohol
replacement by toluene. Then toluene was
replaced by paraffin. Longitudinal
microtome slices were obtained from the
fixed material by paraffin blocks method
[5]. A part of obtained slices were left
unstained, the rest of the slices were stained
by haematoxylin.
For electron-microscopic
examination, nodules of plants inoculated by
R. galegae K-3 were separated, cut into the
layers of 1 mm thickness, fixed with 6 %
glutaric aldehyde solution and 1 % OsO4 [6].
Dehydration of fixed material as performed
by 50, 70, 76 and 100 % alcohols and
poured in Epone-812. Ultrathin slices were
obtained on ultramicrotom BS 490A, stained
with uranyl acetate, examined under
electronic microscope BS-540 “Tesla”.
In the main phase of plant
development of the first and second years of
cultivation, number of nodules on the roots
of plants and activity of symbiotic nitrogen
fixation measured by acetylene method,
were determined [7]. Roots were sampled
from soil-plant monoliths of 22x22x27 cm,
carefully separating from the soil, washed
and placed them in bottles where acetylene
was injected. Incubation time was 1 hour.
Amount of reduced ethylene was measured
on a gas chromatograph Chrom-4. Then the
roots were removed from the bottles, and
number of nodules was counted.
Mowing of plants was on the first
and second year of vegetation of Galega
orientalis in the flowering stage, with further
measurement of their air dry tops.
Statistical processing of experimental
data was carried out by B. O. Dospiekhov
[8] using computer program Statistica 6.0.
Therefore, selected active strain
Rhizobium galegae K-3 forms an effective
nitrogen-fixing symbiosis with plants of
Galega orientalis and promotes formation of
the significant number of nodules with
distinct histological zoning on the roots that
is typical for non-determinated type of
nodules. It has been established that
symbiotic apparatus of Galega orientalis,
formed during the first year, after wintering
retains its structure and functional activity.
During the second year of plants cultivation,
increase in the number of nodules on the
roots of plants and a significant increase in
the level of symbiotic nitrogen fixation is
observed since the beginning of regrowth of
the tops. Mowing of Galega orientalis does
not affect intensity of nodule formation, but
significantly reduces the activity of
symbiotic nitrogen fixation. Strain
Rhizobium galegae K-3 improves
productivity of Galega orientalis by 20 %, in
total of two years of cultivation compared
with control.
|
| id | oai:ojs2.smic.in.ua:article-109 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:00:23Z |
| publishDate | 2016 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/8a/2a3900764399a0aea21891ca54d4048a.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1092026-07-22T10:10:42Z THE FEATURES OF INTERACTION RHIZOBIUM GALEGAE WITH GALEGA ORIENTALIS ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ Воробей, Ю. О. Воробей, В. С. Пиріг, О. В. Rhizobium galegae, Galega orientalis, legume-rhizobial symbiosis Rhizobium galegae, козлятник східний, бобово-ризобіальний симбіоз The strain of Rhizobium galegae К- 3 - active symbiote of Galega orientalis has been received by means of selection. Positive influence of this strain on formation and functioning of symbiosis during the first and second years of cultivation of Galega orientalis plants is shown. R. galegae К- 3 promotes to formation of generous amount of indeterminate nodules on the roots, increase of activity of symbiotic nitrogen fixation and increase in the yield of Galega orientalis. Селекціоновано штам Rhizobium galegae К-3 — активний симбіонт козлятника східного. Показано позитивний вплив даного штаму на формування та функціонування симбіотичного апарату впродовж першого та другого років вирощування рослин козлятника. Встановлено, що R. galegae К-3 сприяє формуванню на коренях рослин великої кількості бульбочок недетермінованого типу, підвищенню активності симбіотичної азотфіксації та урожайності козлятника східного. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2016-10-09 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/109 10.35868/1997-3004.24.9-17 Agricultural microbiology; Vol. 24 (2016): Agriciltural microbiology; 9-17 Сільськогосподарська мікробіологія; Том 24 (2016): Сільськогосподарська мікробіологія; 9-17 1997-3004 10.35868/1997-3004.24 en uk https://smic.in.ua/index.php/journal/article/view/109/107 https://smic.in.ua/index.php/journal/article/view/109/108 Авторське право (c) 2016 Yu. O. Vorobei, V. S. Vorobei, А. V. Piroh https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Rhizobium galegae козлятник східний бобово-ризобіальний симбіоз Воробей, Ю. О. Воробей, В. С. Пиріг, О. В. ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title | ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title_alt | THE FEATURES OF INTERACTION RHIZOBIUM GALEGAE WITH GALEGA ORIENTALIS |
| title_full | ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title_fullStr | ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title_full_unstemmed | ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title_short | ОСОБЛИВОСТІ ВЗАЄМОДІЇ RHIZOBIUM GALEGAE З КОЗЛЯТНИКОМ СХІДНИМ |
| title_sort | особливості взаємодії rhizobium galegae з козлятником східним |
| topic | Rhizobium galegae козлятник східний бобово-ризобіальний симбіоз |
| topic_facet | Rhizobium galegae Galega orientalis legume-rhizobial symbiosis Rhizobium galegae козлятник східний бобово-ризобіальний симбіоз |
| url | https://smic.in.ua/index.php/journal/article/view/109 |
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