СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ
The estimation of nitrogen fixation process intensity in meadow clover plants in symbiosis with Rhizobium leguminosarum bv. trifoliistrain 329б, active agent of Rhyzobofit preparation, was performed. Plant varieties that were characterized with the greatest symbiotic and nodulation ability as well a...
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| Дата: | 2014 |
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| Автори: | , |
| Формат: | Стаття |
| Мова: | Англійська Українська |
| Опубліковано: |
Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Назва журналу: | Agriciltural microbiology |
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Репозитарії
Agriciltural microbiology| _version_ | 1871465609846325248 |
|---|---|
| author | Кічігіна, О. О. Патика, В. П. |
| author_facet | Кічігіна, О. О. Патика, В. П. |
| author_institution_txt_mv | [
{
"author": "О. О. Кічігіна",
"institution": "Інститут агроекології і природокористування НААН"
},
{
"author": "В. П. Патика",
"institution": "Інститут мікробіології і вірусології ім. Д. К. Заболотного НАН України"
}
] |
| author_sort | Кічігіна, О. О. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The estimation of nitrogen fixation process intensity in meadow clover plants in symbiosis with Rhizobium leguminosarum bv. trifoliistrain 329б, active agent of Rhyzobofit preparation, was performed. Plant varieties that were characterized with the greatest symbiotic and nodulation ability as well as seed productivity within the three years were selected. |
| doi_str_mv | 10.35868/1997-3004.20.7-11 |
| first_indexed | 2025-07-17T12:24:08Z |
| format | Article |
| fulltext |
SYMBIOSIS OF RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII WITH
RED CLOVER VARIETIES
O. O. Kichigina1, V. P. Patyka2
1Institute of Agroecology and Environmental Management, NAAS, Kyiv
12, Metrolohichna Str., Kyiv City, 03143, Ukraine e-mail: ol_ki@ukr.net
2Danylo Zabolotny Institute of Microbiology and Virology, NAS of Ukraine, Kyiv
154, Zabolotnoho Str., Kyiv City, 03143, Ukraine
Biological nitrogen is a powerful
factor in improving potential soil fertility,
significant saving of mineral nitrogen
fertilizers, reducing pollution of environment
by harmful nitrogen compounds [7].
More than half of fixed nitrogen on the
planet is bound through functioning the
symbiosis of legumes and nodule bacteria,
and in soil and climatic conditions of Ukraine,
depending on legumes that are grown,
legume-rhizobial systems are able to fix from
40 to 300 kg or more of nitrogen per 1 hectare
of crops from the atmosphere yearly [1; 8;
12]. According to A. O. Babych [10], red
clover shows the productivity of nitrogen
fixation of 115-208 kg of nitrogen/ha per
year, while the proportion of biological
nitrogen in the formation of yield reaches 70-
90%, the balance of nitrogen in the soil – 60-
80 kg/ha.
Thus, red clover, in addition to its
value as a high-protein crop has important
agrotechnical value. It accumulates
biologically fixed nitrogen in the soil,
fertilizes soil due to plant debris, and
improves its structure, being as a result a good
forecrop for all crops. Its use both in pure
sowing and in mixtures with grasses enhances
the productivity and quality of feed [9].
Therefore, at the formation of vegetation
particular attention should be paid to the
introduction of red clover varieties with high
potential for nitrogen fixation into grass
mixtures [3].
It should be taken into consideration
that the interaction between macro- and
micro-symbionts is not always characterized
by high efficiency of fixation of molecular
nitrogen. It is known that the efficiency level
of legume-rhizobial symbiosis is defined by
genotypes of both partners - nodule bacteria
and legume plants-feeders [1; 8; 11].
Polymorphism by the activity of a
symbiotic complex “legume - nodule
bacteria” includes two aspects: variability and
diversity of nodule bacteria strains and
different reactions of a plant-feeder to the
interaction with the bacterial strain.
Therefore, high performance of symbiotic
nitrogen fixation is not possible without
aimed breeding of the varieties of legumes
and complementary strains of nodule bacteria
given specific climatic and agronomic soil
and agrotechnical conditions.
The development of nodule bacteria is
closely related to the growth of legume plants.
Its genotype determines specific energy
supply for the needs of nitrogen-fixing
microorganism as the products of
photosynthesis and root residues, as well as
regulatory, warning and synanthropic
processes of interaction [12]. Therefore, when
red clover varieties are used in agriculture the
features that characterize the ability to fix
atmospheric nitrogen, except for their
commercially valuable ones, should be
considered.
The goal of our study was to estimate
nitrogen fixation process intensity in red
clover plants in symbiosis with Rhizobium
leguminosarum bv. trifolii strain 329b and
allocation of donor genotypes of clover
according to this indicator as well as seed
yield.
Field small-plot experiment with the
varieties of red clover (T. pratense L.) was
conducted at the Institute of Feed of NAAS
(2006-2008). Soil - gray forest podzolic
middle-loam; humus content – 2.06%; pH
4.8; hydrolytic acidity – 3.24 meq./100 g of
soil; amount of absorbed bases - 19.2 mg-
Eq/100 g of soil; content of nitrogen available
for plants - 74 mg/kg of soil (by Kornfield);
labile phosphorus – 173 mg/kg of soil and
exchangeable potassium – 88 mg/kg of soil
(by for Kirsanov). Area of a research plot was
2 m2. Repetition of the experiment - six times.
The study was conducted in the crops
of red clover of the second year of cultivation.
Nitrogenase activity was determined
by using a gas chromatograph “Chrom 5”
with ionizing flame detector [13]. The number
of nodules on the roots of plants – visually by
direct calculation. The study was conducted in
the second week of June.
Collection and recording of seeds
yield was carried out in August.
Detection of the probability of the
obtained results was performed using standard
computer programs “Statistics” by Student
factor – in the laboratory experiments and by
Fischer factor – in the field ones, using the
recommendations of guidebooks on
mathematical analysis of experiment results
[2; 5; 6].
The estimation of nitrogen fixation
process intensity in meadow clover plants in
symbiosis with Rhizobium leguminosarum bv.
trifolii strain 329b, active agent of Rhyzobofit
preparation, was performed. Plant varieties
that were characterized with the greatest
symbiotic and nodulation ability as well as
seed productivity within the three years were
selected.
The increase in Poltava 75 variety
share in the area of forest steppe of Ukraine,
and Anitra, Marusia, Myronivska 5, Milvus,
Nosivska 5, Sparta varieties share in the area
of both forest-steppe and Polissia in the crops
of red clover as varieties with high nitrogen-
fixing potential will help to get feeds of high
quality, solve the problems of shortage of
protein in animal feed, improve soil fertility
and ultimately – save a significant amount of
mineral nitrogen fertilizers that will improve
environmental conditions in agroecosystems
in general.
|
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| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:04:46Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
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| spelling | oai:ojs2.smic.in.ua:article-1522026-07-22T10:10:43Z SYMBIOSIS OF RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII WITH RED CLOVER VARIETIES СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ Кічігіна, О. О. Патика, В. П. clover varieties, biological nitrogen, nitrogen-fixing capacity, soil fertility сорти конюшини лучної, біологічний азот, азотфіксувальний потенціал, родючість ґрунту The estimation of nitrogen fixation process intensity in meadow clover plants in symbiosis with Rhizobium leguminosarum bv. trifoliistrain 329б, active agent of Rhyzobofit preparation, was performed. Plant varieties that were characterized with the greatest symbiotic and nodulation ability as well as seed productivity within the three years were selected. Проведено оцінювання інтенсивності процесу азотфіксації сортів конюшини лучної в симбіозі зі штамом Rhizobium leguminosarum bv. trifolii 329б, нa основі якого виробляється Ризобофіт. Виділено сорти, що впродовж трьох років досліджень характеризувалися найвищою здатністю до симбіозу, нодуляційною активністю та урожайністю насіння. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-12-22 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/152 10.35868/1997-3004.20.7-11 Agricultural microbiology; Vol. 20 (2014): Agriciltural microbiology; 7-11 Сільськогосподарська мікробіологія; Том 20 (2014): Сільськогосподарська мікробіологія; 7-11 1997-3004 10.35868/1997-3004.20 en uk https://smic.in.ua/index.php/journal/article/view/152/189 https://smic.in.ua/index.php/journal/article/view/152/190 Авторське право (c) 2014 O. O. Kichigina, V. P. Patyka https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | сорти конюшини лучної біологічний азот азотфіксувальний потенціал родючість ґрунту Кічігіна, О. О. Патика, В. П. СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title | СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title_alt | SYMBIOSIS OF RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII WITH RED CLOVER VARIETIES |
| title_full | СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title_fullStr | СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title_full_unstemmed | СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title_short | СИМБІОЗ RHIZOBIUM LEGUMINOSARUM BV. TRIFOLII З СОРТАМИ КОНЮШИНИ ЛУЧНОЇ |
| title_sort | симбіоз rhizobium leguminosarum bv. trifolii з сортами конюшини лучної |
| topic | сорти конюшини лучної біологічний азот азотфіксувальний потенціал родючість ґрунту |
| topic_facet | clover varieties biological nitrogen nitrogen-fixing capacity soil fertility сорти конюшини лучної біологічний азот азотфіксувальний потенціал родючість ґрунту |
| url | https://smic.in.ua/index.php/journal/article/view/152 |
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