ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL.
The paper presents the study of the effect of fungicides solutions on the germination of conidia of fungi Cercospora beticola Sacc. and Alternaria alternata (Fr.) Keissl. It was found that inhibition of spore germination with fungicides had significantly depended on the duration of storage and the o...
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| Datum: | 2014 |
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| Назва журналу: | Agriciltural microbiology |
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Agriciltural microbiology| _version_ | 1871465655808557056 |
|---|---|
| author | Власюк, О. С. |
| author_facet | Власюк, О. С. |
| author_institution_txt_mv | [
{
"author": "О. С. Власюк",
"institution": "Хмельницька державна сільськогосподарська дослідна станція Інституту кормів та сільського господарства Поділля НААН"
}
] |
| author_sort | Власюк, О. С. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:43Z |
| description | The paper presents the study of the effect of fungicides solutions on the germination of conidia of fungi Cercospora beticola Sacc. and Alternaria alternata (Fr.) Keissl. It was found that inhibition of spore germination with fungicides had significantly depended on the duration of storage and the origin of the fungal isolates. Conidia of C. beticola were less resistant to the action of fungicides than spores of A. alternata. Fungicides Derozal and Alto Super were shown to be the most effective against the germination of spores of Cercospora leaf spot pathogen, while Alto Super — against the Alternaria spot disease. |
| doi_str_mv | 10.35868/1997-3004.19.61-67 |
| first_indexed | 2025-07-17T12:24:16Z |
| format | Article |
| fulltext |
INHIBITORY ACTION OF FUNGICIDES ON GERMINATION OF FUNGAL
SPORES OF CERCOSPORA BETICOLA SACC. AND ALTERNARIA
ALTERNATA KEISSL.
O. S. Vlasiuk
Khmelnitskiy State Agricultural Experimental Station, Institute of feed research and agriculture of
Podillia of NААS
1, Samchyky Str., Samchyky village, Starokostiantynivskyi district, Khmelnytsk region, 31182,
Ukraine; vchsekretar-hdsgds@yandex.ru
In recent years a clear trend towards
strengthening of cercosporosis and Alternaria
spot disease of sugar beets has been observed,
which affects the yield of crops and the
quality of a raw product for the production of
sugar [1; 2]. Despite the significant amount of
research, there are substantial differences in
the evaluation of the effectiveness of
fungicides against these diseases. Almost
unexplored is sugar beets blight, which had
not been identified before 2000 in our
country.
Cercospora beticola Sacc. fungus
belongs to necrotrophs – pathogens of spot
necroses and is considered highly pathogenic
[3]. The prevalence of the disease and often
epiphytoty are caused by great potential of C.
beticola fungus reproduction. Thus, the
number of conidia per plant with strong
development of spot disease varies from 200
to 500 mln. units [4]. It should be noted that
cercosporosis is one of the most harmful
diseases of sugar beet. Even at low lesions
(less than 1 point) the loss of sugar is 5 to
10%, at average (1.5-2 points) – from 20 to
30%, and at strong (over 2 points) – from 30
to 70% [5]. At this, there are no immune to
cercosporosis varieties or hybrids of sugar
beet (both among feeding and table beets) [6].
Alternaria alternata (Fr.) Keissl.
belongs to fungi that live as saprophytes on
many kinds of plants. They lower the rate of
photosynthesis, reducing the access of light to
tissues and interfering with gas exchange [7].
Under conditions of excessive moisture the
fungus can move from saprophytic form to
parasitic. High humidity (over 96%) and
periodic changes of dry weather with rainy
one at temperatures not below 22°C
contribute to its development [3]. A. alternata
is a secondary pathogen on beets that
penetrates mainly into chlorotic beet leaf
areas, particularly affected by weak chlorosis
virus and debilitated by the lack of macro-
and microelements [8; 9]. In addition, it is
believed that the pathogen is a complex of
species, so-called “pathotype” of many fungi
of Alternaria genus, but the issue today is
uncertain and requires additional studies [10;
11].
Besides the harmfulness to crops,
scientific literature marked mutagenic effect
of A. alternata culture liquid, isolated from
corn in China, in the region with a high level
of human esophageal cancer, on bacteria and
human cells [10].
Due to prolonged use of the same
fungicide the increase of resistance is
observed in the pathogen. Thus, in the USA
the percentage of Cercospora beticola spores
resistant to benzimidazole derivatives was
higher in areas where the preparation was
most often used. Such pathogen resistance
was caused by α-tubulin and β-tubulin genes
mutation under the influence of
benzimidazole. The isolates of that mutation
have an increased sensitivity to
phenylcarbamate [12; 13]. In Hungary, the
resistance of Alternaria species to benomyl
was found, which greatly accelerated the
drying of beet leaves [14].
High degree of variability in
cercospora isolates promotes the increasing of
resistance to fungicides. Thus, in Greece
among 45 isolates from four beet-growing
areas 45 pathogen genotypes of cercosporosis
were identified, which differ with variability,
preferably, within a population [15].
Rapid development of cercospora
strains tolerant to fungicides, is explained by
high adaptive capacity of the fungi of that
genus [16]. In addition, it was studies that low
application rate of some fungicides, especially
Kuproksil, Benlat and Topsin act as mutagens
to C. beticola conidia, and single mutants
have much higher virulence compared to the
isolates of fungus natural type [17].
Due to the urgency of the issue and
conflicting data on the effectiveness of
fungicides we studied the impact of
fungicides solutions on the germination of
conidia of fungi-pathogens of cercosporosis
and Alternaria spot disease of sugar beets
depending on the duration of storage and the
origin of the isolates.
Materials and methods. For
comparative evaluation of sustainability of
Cercospora beticola Sacc. and Alternaria
alternata (Fr.) Keissl. conidia to storage and
influence of fungicides studied was the effect
of 0.1% and 0.01% solution (by active
substance) of fungicides on the germination
of conidia obtained from leaves affected by
spot disease that were stored for 6 months in
dry state and from stains on the living leaves.
Laboratory studies fungitoxicity of
preparations were carried by the method of
germination of spores on glass slides [18].
The suspension of fungi spores was
prepared by washing conidia out from beet
leaves after the exposure in a humidified
chamber for amplifying the sporification.
Required preparation concentrations (0.1%
and 0.01%) were received by 20-200 times
dilution of 2% stock solution (by active
substance) with distilled water.
The identification of isolated fungi
was carried out by cultural-morphological
characteristics [19].
The percentage of inhibition of C.
beticola fungus conidia germination was
calculated by Abbott formula [18]:
Т = (Пк По)×
Пк
, (1)
where Т – percentage of spores germination
inhibition, %;
Пк – percentage of germinated
sporesin control, %;
По – percentage of germinated spores
in preparation solution, %.
Concentration equalization by active
substance was carried out according
to the equation:
Concentration of preapration (%) =
= ×
(%)
(2)
In vitro was performed the
determination of braking action of fungicides
solutions: Impact 25, SC, concentrate of
suspension, (25% flutriaphol) – 0.25 l/ha, Rex
Top, concentrate of suspension, (25%
phenpropimorph + 8.4% epoxiconazole) – 0.8
l/ha, Alto Super 330 EC, concentrate of
emulsion, (8% cyproconazole 8% + 25%
propiconazole) – 0.4 l/ha Derozal concentrate
of suspension, (50% carbendazim) – 0.5 l/ha
and Subtan, liquid paste, (analogue – Agat-
25K, inactivated bacteria Pseudomonas
aureofaciens 2% and products of their
metabolism 38%) – 0.15 l/ha on germination
of pathogens conidia of cercosporosis and
Alternaria spot disease of sugar beets. Hectare
preparation standard contains Impact 25 SC –
62.5 g of active ingredient, Subtan – 60 g,
Alto Super – 165 g, Derozal – 200 g and Rex
Top – 233.8 g of active ingredient, the
concentration of working solutions is
corresponding. Braking action of these
preparations on the germination of spot
disease pathogens spores at equal
concentration by active substance was
determined.
So, C. beticola fungus conidia are less
resistant to fungicides than A. Alternate
spores, which indicates possible problems in
controlling Alternaria spot disease of sugar
beets in the future.
The effectiveness of inhibition of
cercosporosis pathogen conidia germination
by fungicides is reduced under processing of
spores from vegetative plants, compared with
previous year's models.
Effective against the germination of
spores of cercosporosis fungus-pathogen are
fungicides Derozal and Alto Super, against
Alternaria spot disease of sugar – Alto Super.
Least of all the germination of conidia of both
pathogens was inhibited by biofungicide
Subtan. Although fungi hyphae were
practically not viable in fungicide solutions,
the chance for appearance of resistant to
certain preparations isolates of C. beticola
Sacc. and A. alternata (Fr.) Keissl., especially
at low concentrations of fungicides, is quite
high.
The isolates of C. beticola and A.
alternata from Khmelnytskyi SAES and
isolates from Ivanivka ESS exhibit varying
resistance to fungicides activity. These data
should be considered in further studies of the
biology of these pathogens and in the practice
of fungicides application against them.
|
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| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:05:29Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
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| resource_txt_mv | smicinua/dc/67266b848498c3bc7495a11a748ae8dc.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1722026-07-22T10:10:43Z INHIBITORY ACTION OF FUNGICIDES ON GERMINATION OF FUNGAL SPORES OF CERCOSPORA BETICOLA SACC. AND ALTERNARIA ALTERNATA KEISSL. ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. Власюк, О. С. Cercospora beticola Sacc., Alternaria alternata (Fr.) Keissl., fungicides, sugar beet, isolate Cercospora beticola Sacc., Alternaria alternata (Fr.) Keissl., фунгіциди, буряки цукрові, ізолят The paper presents the study of the effect of fungicides solutions on the germination of conidia of fungi Cercospora beticola Sacc. and Alternaria alternata (Fr.) Keissl. It was found that inhibition of spore germination with fungicides had significantly depended on the duration of storage and the origin of the fungal isolates. Conidia of C. beticola were less resistant to the action of fungicides than spores of A. alternata. Fungicides Derozal and Alto Super were shown to be the most effective against the germination of spores of Cercospora leaf spot pathogen, while Alto Super — against the Alternaria spot disease. Наведено результати досліджень впливу розчинів фунгіцидів на проростання конідій грибів Cercospora beticola Sacc. та Alternaria alternata (Fr.) Keissl. Встановлено, що пригнічення проростання спор фунгіцидами залежало від тривалості зберігання та походження ізолятів грибів. Конідії гриба C. beticola менш стійкі до дії фунгіцидів, ніж спори A. аlternata. Найбільше пригнічують проростання спор гриба-збудника церкоспорозу фунгіциди Дерозал та Альто Супер, альтернаріозу — Альто Супер. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-06-26 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/172 10.35868/1997-3004.19.61-67 Agricultural microbiology; Vol. 19 (2014): Agriciltural microbiology; 61-67 Сільськогосподарська мікробіологія; Том 19 (2014): Сільськогосподарська мікробіологія; 61-67 1997-3004 10.35868/1997-3004.19 en uk https://smic.in.ua/index.php/journal/article/view/172/227 https://smic.in.ua/index.php/journal/article/view/172/228 Авторське право (c) 2014 O. S. Vlasiuk https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | Cercospora beticola Sacc. Alternaria alternata (Fr.) Keissl. фунгіциди буряки цукрові ізолят Власюк, О. С. ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title | ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title_alt | INHIBITORY ACTION OF FUNGICIDES ON GERMINATION OF FUNGAL SPORES OF CERCOSPORA BETICOLA SACC. AND ALTERNARIA ALTERNATA KEISSL. |
| title_full | ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title_fullStr | ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title_full_unstemmed | ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title_short | ГАЛЬМУЮЧА ДІЯ ФУНГІЦИДІВ НА ПРОРОСТАННЯ СПОР ГРИБІВ CERCOSPORA BETICOLA SACC. ТА ALTERNARIA ALTERNATА KEISSL. |
| title_sort | гальмуюча дія фунгіцидів на проростання спор грибів cercospora beticola sacc. та alternaria alternatа keissl. |
| topic | Cercospora beticola Sacc. Alternaria alternata (Fr.) Keissl. фунгіциди буряки цукрові ізолят |
| topic_facet | Cercospora beticola Sacc. Alternaria alternata (Fr.) Keissl. fungicides sugar beet isolate Cercospora beticola Sacc. Alternaria alternata (Fr.) Keissl. фунгіциди буряки цукрові ізолят |
| url | https://smic.in.ua/index.php/journal/article/view/172 |
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