ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ
The influence of seed protectants crop viability and functional activity of bacterial cells - biological agents microbial preparations. The possibility of the use of protectants compatible with biological products, bio-agents which exhibit resistance to the pesticide active ingredient.
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Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine
2014
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| author | Токмакова, Л. М. Тараріко, Ю. О. Трепач, А. О. Лепеха, О. П. Ларченко, І. В. |
| author_facet | Токмакова, Л. М. Тараріко, Ю. О. Трепач, А. О. Лепеха, О. П. Ларченко, І. В. |
| author_institution_txt_mv | [
{
"author": "Л. М. Токмакова",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "Ю. О. Тараріко",
"institution": "Інститут водних проблем і меліорації НААН"
},
{
"author": "А. О. Трепач",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "О. П. Лепеха",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "І. В. Ларченко",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Токмакова, Л. М. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:44Z |
| description | The influence of seed protectants crop viability and functional activity of bacterial cells - biological agents microbial preparations. The possibility of the use of protectants compatible with biological products, bio-agents which exhibit resistance to the pesticide active ingredient. |
| doi_str_mv | 10.35868/1997-3004.18.120-131 |
| first_indexed | 2025-07-17T12:24:22Z |
| format | Article |
| fulltext |
1
EFFECTS OF MODERN SEED PROTECTANTS ON VIABILITY AND FUNCTIONAL
ACTIVITY OF BIOLOGICAL AGENTS OF MICROBIAL PREPARATIONS
Tokmakova L.М.1, Tararyko Yu.О.2, Trepach А.O.1, Lepekhа О.P.1 , Larchenko І.V.1
1Institute of Agricultural Microbiology and Agroindustrial Production NAAS,
97, Shevchenko str., Chernihiv, 140027, Ukraine
2Institute of water problems and land reclamation of NAAS
37, VAsylkivska str., Kyiv, 03022, Ukraine
e-mail: tokmakova_ln@mail.ru
The influence of seed protectants crop viability and functional activity of bacterial cells -
biological agents microbial preparations. The possibility of the use of protectants compatible with
biological products, bio-agents which exhibit resistance to the pesticide active ingredient.
Keywords: microbial drugs, viability of microorganisms, insecticides, fungicides.
It is known that the level of crop yields greatly depends on the phytosanitary status of crops. Thus,
according to the FAO, the global agriculture from pests is lost at least a third of the crop, and during mass
breeding crop dies almost completely. In Ukraine, the average loss of production of major crops from
pests are 25 – 30%, including: wheat – 27%, corn – 29%, sugar beet – 27% [1]. Therefore, the use of
plant protection products is an integral part of modern technologies of growing crops.
At the same time, the use of fungicides and insecticides for pre-treatment of seeds is not always
reasonable due to their incompatibility with bacterial preparations of aimed on the improvement of soil
fertility which use is becoming more common in crops growing practices [2]. At seed treatment its
surface becomes an interaction area of bacteria with a chemical agent which toxicity can considerably
reduce the effect of inoculation. Thus the current study was focused on the opportunities study of
beneficial microorganisms introduction into the agrocenosis at use of modern pesticides for seeds
treatment.
The purpose of given research was to determine the viability and functional activity of bacterial
cells – biological agents of microbial preparations under the actions of protectants.
Materials and methods. The study was performed using the following bacteria: Bradyrhizobium
japonicun M-8 (bio-agent of Rhizohumin); Paenibacillus polymyxa EF (bio-agent of Polimixobakteryn);
Achromobacter album 1122 (bio-agent of Albobakteryn); Azospirillum brasilense 410 (bio-agent of
Microhumin); fungicides: Alpha protectant, t.k.s. (tebuconazole, 60 g/l + imazalil, 100 g/l; UK), Alpha
Epoxyl (epoxiconazole, 187 g/l + thiophanate-methyl, 310 g/l, UK), Absolute (carbendazim, 500 g/l,
Ukraine), Alios (triticonazole, 300 g/l, Germany), Viale Trust (tebuconazole, 60 g/l + thiabendazole,
2
80 g/l, Russia), Viale TT (tebuconazole 60 g/l + thiabendazole 80 g/l, Russia), Vinets (flutriafol, 30 g/l +
thiabendazole, 45 g/l, Ukraine), Gizmo 60 (tebuconazole, 60 g/l, Austria), Colfugo Super (carbendazim,
200 g/l, Ukraine), Corriolis (triticonazole, 200 g/l, Switzerland), Forsazh 500 Sc (carbendazim, 500 g/l,
Ukraine), Funaben T 480 (tiuram, 332 g/l + carbendazim, 148 g/l, Poland); and insecticides: Celeste Top
312,5 Fs (difenoconazole, 25 g/l + fludioxonil, 25 g/l + tiametoxam, 262.5 g/l, 700 g/kg, Switzerland),
Tabooo (imidacloprid, 500 g/l, Russia).
Protectants were sterilized with diethyl ether in 15 cm3 bottles with a cotton stopper. Sterility
verification was performed by sowing aqueous suspension of protectants in culture medium. Qualitative
responses of bacteria – bio-agents of microbial preparations on the fungicides action was determined
using paper discs [3]. The resistance degree of bacterial strains to protectants was determined using
Zibalskiy method [4]. The titer of bacterial cells in suspensions and liquid microbial preparations was
determined by using Goryaeva camera [5]. Dissolving activity of phosphorus compounds by
microorganisms was determined colorimetrically by the Erdei method [6].
Doses of microbial agents and protectants used for seeds treatment were selected in accordance to
the label instructions.
Results and discussion. The effect of modern fungicides Gizmo 60 and Colfugo Super, Forsazh
500 Sc, used for treatment of barley seeds on the viability of Azospirillum brasilense 410 was studied
using paper disks. Sterile fungicide suspensions with sterile water were used in the following ratio: gizmo
60 : water – 0.5:10; Super Colfugo : water – 3.0: 10; Forsazh 500 Sc : water – 1.2: 10. It was shown that
neither of the studied fungicides had affected the growth of Az. brasilense 410.
Cell viability of Az. brasilense 410 under the influence of fungicides Gizmo 60, Colfugo Super,
Forsazh 500 Sc was examined in the work solution consisted of Mikrohumin and protectants in the
following ratio: Gizmo 60 : Mikrohumin – 0.5:10; Colfugo Super : Mikrohumin – 3.0:10, Forsazh 500 Sc
: water – 1.2:10. On the next day the filter paper disk was moistened in the working mixture and placed
on nutrient potato agar medium followed by the plates’ examination on the presence or absence of
bacteria biomass growth around the disc. It was established that examined protectants had no effect on the
cell viability of Az. brasilense 410 grown in the in the working mixture used for seeds treatment.
The data obtained indicates the possibility of the simultaneous use of Mikrohumin and protectants
Gizmo 60, Colfugo Super and Forsazh 500 Sc in spring barley growing technologies.
No adverse effects on cell growth of Br. japonicun M-8 were observed under the influence of
Alpha protectant (no growth retardation zone was observed around the paper disk moistened with
protectant) (Figure 1). Alpha Epoxyl on the other hand had shown a negative effect on cell growth of Br.
japonicum M-8 – the 11 mm zone of growth retardation was formed around the paper disk impregnated
with protectant (Fig. 2).
3
Figure 1. Effect of Alpha Protectant on
the cell growth of Br. japonicum M-8
using paper disks (paper disk is
moistened in water suspension of water :
Alpha Protectant – 10:0.5)
Figure 2. Effect of Alpha Epoxyl on the
cell growth of Br. japonicum M-8 using
paper disks (paper disk is moistened in
water suspension of water : Alpha Epoxyl
– 10:0.6)
Cell viability of Br. japonicum M-8 in the working mixture under the influence of fungicide Alpha
Epoksyl was not determined due to the negative effect it has on the growth of bacteria.
Effect of Alpha Protectant on cell viability of Br. japonicum M-8 was investigated in the working
mixture of bacterial suspension and protectant in the ratio of 0.5:10. In 24 hours the filter paper disks
moistened in working mixture were placed on a nutrient agar medium with pea broth. The presence or
absence of the bacterial growth was used to determine the level of influence. It was revealed that Alpha
Protectant does not adversely affect the cell viability of Br. japonicum M-8 in the working mixture used
for treatment of soybean seeds (the growth of bacterial biomass was observed around the disc moistened
in the working solution) (Fig. 3).
4
Figure 3. Effect of fungicide Alpha
Protectant on the cell growth of Br.
japonicum M-8 using paper disks (paper
disk is moistened in water suspension of
water : Alpha Protectant – 10:0.5)
The results obtained have showed that fungicide Alpha Protectant had no toxic effect on the
growth and development of cells Br. japonicun M-8 – bio-agents of microbial preparation Rhizohumin,
indicating the possibility of their use in soybean growing technology.
Bacteria Br. japonicum M-8 does not remain viable in the working mixture of fungicide Alpha
Epoxyl used for treatment of soybean seeds, thus excluding their combined use.
The action of modern fungicide Funaben T 480 on viability of Achr. album 1122 cells was studied
using the product suspension prepared in accordance to the recommendations of rapeseeds treatment. For
this the sterilized fungicide and sterile water were used in the ratio of 2.5:10. It was established that
fungicide Funaben T 480 had no toxic effect on cell growth of bacteria Achr. album 1122.
Cell viability of Achr. album 1122 was investigated using the working mixture consisted of
bacterial suspension and protectant in the ratio of 2.5:10. In 24 hours the filter paper disk moistened in
working mixture was placed on nutrient agar medium MPA and in a few days the presence or absence of
bacterial growth around the disc was determined. The studies have shown that fungicide Funaben T 480
had no effect on the viability of Achr. album 1122 used for treatment of rapeseed (the growth of bacterial
biomass was observed around the disc) thus indicating the possibility of simultaneous use of Achr. album
1122 and Funaben T 480 in rape growing technologies.
The same methods of paper disks was used for investigation of the effect of modern fungicides:
Absolute, Alios, Gizmo 60, Colfugo Super, Corriolis, Vial Trust, Vial TT, Vinets, Forsazh 500 Sc,
Funaben T 480 and insecticides: Celeste Top 312,5 Fs, Taboo on the viability of P. polymyxa KB. The
working suspensions were made of sterilized fungicide (used at label recommendations) and sterile water
in the following ratio: Absolut:water – 2.0:10; Alios:water – 2.0: 10; Vinets:water – 1.8:10; Gizmo
60:water – 0.5:10; Colfugo Super:water – 3.0:10; Corriolis:water – 0.2:10; Vial Trust:water – 3.0:10; Vial
TT:water – 0.15:10; Celeste Top 312,5 Fs:water – 2,0:10 Tabooo:water – 6,0:10; Forsazh 500 Sc:water –
5
1.2:10; Funaben T 480:water – 2.5:10. The experiments performed have showed that studied fungicides
had no effect on growth of P. polymyxa KB.
Viability of P. polymyxa KB cells was studied using the working mixtures consisted of bacterial
suspension and protectants in the following ratio: Absolut:Polymyxobacteryn – 2.0:10;
Alios:Polymyxobacteryn – 2.0:10; Vinets:Polymyxobacteryn – 1.8: 10; Gizmo 60:Polymyxobacteryn –
0.5:10; Colfugo Super:Polymyxobacteryn – 3.0:10; Corriolis:Polymyxobacteryn – 0.2:10; Vial
Trust:Polymyxobacteryn – 3.0:10; Vial TT:Polymyxobacteryn – 0.15:10; Celeste Top 312,5
Fs:Polymyxobacteryn – 2.0:10 Taboo:Polymyxobacteryn – 6.0: 10; Forsazh 500 Sc:Polymyxobacteryn –
1.2:10; Funaben T 480:Polymyxobacteryn – 2.5:10. After day exposure in the working mixture filter
paper discs were placed on a potatoes nutrient agar medium. Plates were then checked for the presence or
absence of bacterial growth around the disc. It was established that fungicides Absolute, Alios, Vinets,
Gizmo 60, Colfugo Super, Corriolis, Vial Trust, Vial TT, Forsazh 500 Sc, Funaben T 480 and
insecticides: Celeste Top 312,5 Fs, Taboo had no effect on the viability of P. polymyxa KB in the working
mixture used for seeds treatment (bacterial growth around the discs moistened in the working mixture was
observed).
Bacteria P. polymyxa KB remain their viability and functional activity in the working mixture of
fungicides Absolute, Alios, Vial Trust, Vial TT, Vinets, Gizmo 60, Colfugo Super, Corriolis, Forsazh 500
Sc, Funaben T 480 and insecticide Celeste Top Fs 312.5, Taboo which indicates the possibility of their
joint use and Polymyxobacteryn for seed treatment of sugar beet seeds, spring and winter wheat, barley,
sunflower, corn, canola (Table. 1).
The results obtained may be projected on the other pesticides with the same active substances and
their use in the crops growing technologies in combination with bacterial preparations.
Table 1. Effect of seed protectants on growth and viability of Paenіbacіllus polymyxa KB
Protectants
[active ingredients, concentration]
Crop
Bacteria viability
On paper disks
In working
suspension
Fungicides
Absolute
[carbendazim, 500 g/l] Spring wheat + +
Alios
[triticonazole, 300 g/l] Corn + +
Vial Trust [tebuconazole,
60 g/l + thiabendazole, 80 g/l] Winter wheat + +
Vial TT [tebuconazole
60 g/l + thiabendazole 80 g/l] Winter wheat + +
Vinets [flutriafol, 30 g/l
+ thiabendazole, 45 g/l] Cereals + +
6
Gizmo 60
[tebuconazole, 60 g/l] Spring and winter wheat + +
Colfugo Super
[carbendazim, 200 g/l]
Spring and winter wheat,
sunflower + +
Corriolis
[triticonazole, 200 g/l] Winter wheat, corn + +
Forsazh 500 Sc
[carbendazim, 500 g/l] Winter wheat, sunflower + +
Funaben Т 480 [tiuram,
332 g/l + carbendazim, 148 g/l] Spring and winter wheat + +
Insecticides
Celest Top 312,5 Fs
[difenoconazole, 25 g/l + fludioxonil,
25 g/l + tiametoxam, 262,5 g/l]
Winter wheat + +
Taboo [imidacloprid, 500 g/l] Sugar beets, Spring and
winter wheat, rape + +
Note: “+” – no effect.
The success of the combined application of microbial preparations and protectants in crop growing
technologies is guaranteed if fungicides and insecticides do not affect the functional activity of bacteria –
bio-agents of microbial preparations. In this regard, we studied the activity of P. polymyxa KB and Achr.
album 1122 to dissolve phosphates in liquid nutrient medium of Muromtsev under the influence of seed
protectants.
It was established that P. polymyxa KB had retained its functional activity in medium with
fungicides Absolute, Alios, Gizmo 60, Colfugo Super, Corriolis, Vial Trust, Vial TT, Vinets, Forsazh 500
Sc, Funaben T 480 and insecticides Celeste Top 312,5 Fs and Taboo. Phosphates dissolution by bacteria
P. polymyxa KB in a liquid nutrient medium of Muromtsevwith seed protectants had remained on the
same level as in control without pesticides (Table 2).
Table 2. The rate of phosphates dissolution by Paenibacillus polymyxa КВ bacteria in liquid
nutrient medium under the influence of seed protectants
Variants
Amount of phosphorous,
on Р2О5 basis, mg/100 ml*
Paenibacillus polymyxa КВ, control 52,0
Paenibacillus polymyxa КВ at combined cultivation with fungicides:
Absolute 51,8
Alios 51,7
Vial Trust 51,4
Vial TT 51,3
7
Vinets 51,5
Gizmo 60 51,6
Colfugo Super 51,9
Corriolis 51,8
Forsazh 500 Sc 51,8
Funaben Т 480 51,6
Paenibacillus polymyxa КВ at combined cultivation with insecticides:
Celest Top 312,5 Fs 51,3
Taboo 51,7
*) liquid nutrient medium of Muromtsev was used with Са3(РО4)2 – 5 g/l (229 mg Р2О5/100 ml)
Addition of fungicide Funaben T 480 into the liquid nutrient medium of Muromtsev had not
affected the rates of phosphate dissolving by bacteria Achr. album 1122 which was 82.9 mg P2O5 / 100 ml
while the control values were 83.5 mg P2O5 / 100 ml thus indicating that studied protectant had no toxic
effect on the functional activity of bacteria Achr album 1122 (Table 3).
Table 3. The rate of phosphates dissolution by Achromobacter album 1122 bacteria in liquid
nutrient medium under the influence of seed protectants
Variants
Amount of phosphorous,
on Р2О5 basis, mg/100 ml*
liquid nutrient medium of Muromtsev was used with Са3(РО4)2 – 5 g/l (229 mg Р2О5/100 ml)
Achromobacter album 1122 83,5
Achromobacter album 1122 + Funaben Т 480 82,9
Summarizing the performed studies it can be concluded that combined application of protectants
and microbial preparations is possible in the crop growing technologies if bacteria – bio-agents of specific
preparations are resistant to the active ingredients of pesticides.
|
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| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English Ukrainian |
| last_indexed | 2026-07-23T01:06:09Z |
| publishDate | 2014 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
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| resource_txt_mv | smicinua/20/c3f0d886b796b65c7d40e8f13c812020.pdf |
| spelling | oai:ojs2.smic.in.ua:article-1892026-07-22T10:10:44Z EFFECTS OF MODERN SEED PROTECTANTS ON VIABILITY AND FUNCTIONAL ACTIVITY OF BIOLOGICAL AGENTS OF MICROBIAL PREPARATIONS ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ Токмакова, Л. М. Тараріко, Ю. О. Трепач, А. О. Лепеха, О. П. Ларченко, І. В. microbial drugs, viability of microorganisms, insecticides, fungicides мікробні препарати, життєздатність мікроорганізмів, інсектициди, фунгіциди The influence of seed protectants crop viability and functional activity of bacterial cells - biological agents microbial preparations. The possibility of the use of protectants compatible with biological products, bio-agents which exhibit resistance to the pesticide active ingredient. Досліджено вплив протруйників насіння сільськогосподарських культур на життєздатність та функціональну активність клітин бактерій — біологічних агентів мікробних препаратів. Показана можливість сумісного застосування протруйників з біопрепаратами, біоагенти яких проявляють резистентність до діючої речовини пестицидів. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2014-03-31 Article Article Рецензована Стаття application/pdf application/pdf https://smic.in.ua/index.php/journal/article/view/189 10.35868/1997-3004.18.120-131 Agricultural microbiology; Vol. 18 (2014): Agriciltural microbiology; 120-131 Сільськогосподарська мікробіологія; Том 18 (2014): Сільськогосподарська мікробіологія; 120-131 1997-3004 10.35868/1997-3004.18 en uk https://smic.in.ua/index.php/journal/article/view/189/261 https://smic.in.ua/index.php/journal/article/view/189/262 Авторське право (c) 2014 L.М. Tokmakova , Yu.О. Tararyko , А.O. Trepach , О.P. Lepekhа , І.V. Larchenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | мікробні препарати життєздатність мікроорганізмів інсектициди фунгіциди Токмакова, Л. М. Тараріко, Ю. О. Трепач, А. О. Лепеха, О. П. Ларченко, І. В. ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title | ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title_alt | EFFECTS OF MODERN SEED PROTECTANTS ON VIABILITY AND FUNCTIONAL ACTIVITY OF BIOLOGICAL AGENTS OF MICROBIAL PREPARATIONS |
| title_full | ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title_fullStr | ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title_full_unstemmed | ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title_short | ДІЯ СУЧАСНИХ ПРОТРУЙНИКІВ НАСІННЯ СІЛЬСЬКОГОСПОДАРСЬКИХ КУЛЬТУР НА ЖИТТЄЗДАТНІСТЬ ТА ФУНКЦІОНАЛЬНУ АКТИВНІСТЬ БІОЛОГІЧНИХ АГЕНТІВ МІКРОБНИХ ПРЕПАРАТІВ |
| title_sort | дія сучасних протруйників насіння сільськогосподарських культур на життєздатність та функціональну активність біологічних агентів мікробних препаратів |
| topic | мікробні препарати життєздатність мікроорганізмів інсектициди фунгіциди |
| topic_facet | microbial drugs viability of microorganisms insecticides fungicides мікробні препарати життєздатність мікроорганізмів інсектициди фунгіциди |
| url | https://smic.in.ua/index.php/journal/article/view/189 |
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