THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS
The paper covers the study of direct impact and after-effect of 40 t/ha of cattle manure on theorientation of nitrogen fixation and biological denitrification processes in the root zone of potatoes,spring barley, pea, and winter wheat plants in rotation in a stationary field experiments on leachedbl...
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Agriciltural microbiology| _version_ | 1871465543405404160 |
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| author | Volkohon, V. V. Moskalenko, A. M. Dimova, S. B. Zhurba, M. A. Volkohon, K. I. Tokmakova, L. M. Shtanko, N. P. Lutsenko, N. V. |
| author_facet | Volkohon, V. V. Moskalenko, A. M. Dimova, S. B. Zhurba, M. A. Volkohon, K. I. Tokmakova, L. M. Shtanko, N. P. Lutsenko, N. V. |
| author_institution_txt_mv | [
{
"author": "V. V. Volkohon",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "A. M. Moskalenko",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "S. B. Dimova",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "M. A. Zhurba",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "K. I. Volkohon",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "L. M. Tokmakova",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "N. P. Shtanko",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
},
{
"author": "N. V. Lutsenko",
"institution": "Інститут сільськогосподарської мікробіології та агропромислового виробництва НААН"
}
] |
| author_sort | Volkohon, V. V. |
| baseUrl_str | https://smic.in.ua/index.php/journal/oai |
| collection | OJS |
| datestamp_date | 2026-07-22T10:10:41Z |
| description | The paper covers the study of direct impact and after-effect of 40 t/ha of cattle manure on theorientation of nitrogen fixation and biological denitrification processes in the root zone of potatoes,spring barley, pea, and winter wheat plants in rotation in a stationary field experiments on leachedblack soil. Application of manure had significantly increased the nitrogen fixation activity, whilepromoting a high level of N2O emission. The use of microbial preparations for pre-seeding bacterization of seeds optimizes the course of biological nitrogen transformation process — through theenhancement of nitrogen fixation activity and reduction of gaseous nitrogen losses (with the exception of Biogran use on potatoes in the year of manure application). Introduction with manure of alarge number of microorganisms to the soil offsets the positive effect of biopreparations use. Yieldrecords and estimation of grain output per hectare within the crop rotation cycle indicates the practicability of combined application of manure and microbial preparations (excluding the year of direct effect of organic fertilizer) in organic agriculture. |
| doi_str_mv | 10.35868/1997-3004.25.18-24 |
| first_indexed | 2025-07-17T12:23:52Z |
| format | Article |
| fulltext |
18
ҐРУНТОВА МІКРОБІОЛОГІЯ
Сільськогосподарська мікробіологія. — 2017. — Вип. 25. — С. 18–24.
ISSN 1997-3004
UDC 579.64+631.86/87
THE ORIENTATION OF BIOLOGICAL NITROGEN
TRANSFORMATION PROCESSES IN SOIL UNDER THE
ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS
V. V. Volkohon, A. M. Moskalenko, S. B. Dimova, M. A. Zhurba,
K. I. Volkohon, L. M. Tokmakova, N. P. Shtanko, N. V. Lutsenko
Institute of Agricultural Microbiology and Agroindustrial Manufacture, NAAS
97, Shevchenka str., Chernihiv, 14027, Ukraine; e-mail: rifam@ukrpost.ua
The paper covers the study of direct impact and after-effect of 40 t/ha of cattle manure on the
orientation of nitrogen fixation and biological denitrification processes in the root zone of potatoes,
spring barley, pea, and winter wheat plants in rotation in a stationary field experiments on leached
black soil. Application of manure had significantly increased the nitrogen fixation activity, while
promoting a high level of N2O emission. The use of microbial preparations for pre-seeding bacteri-
zation of seeds optimizes the course of biological nitrogen transformation process — through the
enhancement of nitrogen fixation activity and reduction of gaseous nitrogen losses (with the excep-
tion of Biogran use on potatoes in the year of manure application). Introduction with manure of a
large number of microorganisms to the soil offsets the positive effect of biopreparations use. Yield
records and estimation of grain output per hectare within the crop rotation cycle indicates the prac-
ticability of combined application of manure and microbial preparations (excluding the year of di-
rect effect of organic fertilizer) in organic agriculture.
Key words: organic agriculture, microbial preparations, nitrogen fixation, N2O emission,
potato, barley, pea, wheat.
Nowadays we observe an active transition
of the Ukrainian agricultural sector towards the
sustainable and environmentally safe agricul-
ture, including organic production. Thus, at the
beginning of 2003 only 31 farms in Ukraine
were registered as “organic”, while in 2013 their
number was already 175, with the 393.4 thou-
sand hectares of total certified organic agricul-
tural land [1].
The question of the effectiveness of organic
agriculture is reflected in the studies of local
scientists and experts: S. S. Antonets [2],
V. M. Pysarenko [3], M. K. Shykulin [4], and
others. At the same time, many issues related to
the improvement of the efficiency of organic
products manufacture remain unstudied.
The list of factors that influence crops pro-
ductivity in organic agriculture is limited due to
high legislation requirements on hygienic purity
of products. This stipulates the search for agri-
cultural practices that will contribute to the op-
timization of plant root nutrition and crop pro-
duction processes. The methods used for regula-
tion of microbiological activity in agrocoenoses
may be used as the basis for given approach.
Manure is considered to be the one of the most
important factors influencing the formation of
soil microorganisms and the processes they exe-
cute. Manure introduces a huge number of mi-
croorganisms into the soil, and as V. V. Doku-
chaev have said at the end of the nineteenth cen-
tury: “Of course, the bacteria are introduced to
the soil with manure, and their value is probably
not less than in fertilizers” [5]. Microbial prepa-
rations are also a powerful factor of optimiza-
tion of microorganisms’ composition and bio-
logical processes in the soil.
In this regard, the objective of the given
work is to study the orientation of individual
biological processes in agrocenoses under the
use of manure and microbial preparations.
Materials and methods. The study was
© V. V. Volkohon, A. M. Moskalenko, S. B. Dimova, M. A. Zhurba, K. I. Volkohon, L. M. Tokmakova, N. P. Shtanko, N. V. Lutsenko, 2017
19
conducted during 2012–2015 in the stationary
field experiment of the Institute of Agricultural
Microbiology and Agroindustrial Manufacture
NAAS, on leached black soils (pHsalt — 5.30;
humus content — 2.12; easily hydrolized nitro-
gen — 95.2 mg/kg; phosphorus — 226 mg/kg;
exchange potassium — 108 mg/kg). The follow-
ing short crop rotational system was used in the
experiment: potato (variety Belaroza) / spring
barley (variety Gonar) / pea (variety Deviz) /
winter wheat (variety Sonechko).
The impact of 40 t/ha of cattle manure and
microbial preparations authorized for use in or-
ganic production was tested as the crop fertiliza-
tion agents. The following preparations were
used for pre-seeding treatment: Biogran (micro-
bial agent Azospirillum brasilense) was used for
potatoes, Microhumin (microbial agent Azospi-
rillum brasilense) — for spring barley, Rhizo-
humin (microbial agent Rhizobium leguminosa-
rum) — for pea and Polymyxobacterin (micro-
bial agent Paenibacillus polymyxa) — for win-
ter wheat [6].
The potential activity of nitrogen fixation
processes [7] and biological denitrification in
the rhizosphere soil of plants was determined in
dynamics using the acetylene method [8]. The
activity of symbiotic nitrogen fixation in the
root nodules of pea was determined using the
acetylene method [9]. The crops yield was mea-
sured and used for estimation of grain output
per hectare within the crop rotation cycle to
generalize the effect of the investigated factors
on crops productivity.
The statistical processing of the obtained
results was performed using the analysis of
variance [10] and software package Microsoft
Office Excel 2007.
Results and discussion. The study of the po-
tential nitrogen fixation activity in the rhizo-
sphere soil of potato plants indicated the signifi-
cant increases of indices in variants with pplica-
tion of microbial preparation Biogran (Table 1).
However, the activity of the process in-
creases at a greater extent with the use of 40 t/ha
of cattle manure. Application of Biogran com-
bined with the organic background had not
changed the nitrogen fixation activity. These re-
lations were confirmed by the following years
results. Such peculiarity, in our opinion, is ex-
plained by the fact that a large number of mi-
croorganisms, including these that fix atmos-
pheric nitrogen, is introduced to the soil with
Table 1. The effect of organic fertilizer and
bacterization on the potential nitrogenase ac-
tivity of rhizosphere soil of potato plants, 2015
Variants of
experiment
Nitrogenase activity,
nmol С2Н4 / g of dry soil per hour
Start of
budding
stage
Flower-
ing stage
Top
necrosis
stage
without bacterization
Control,
without
fertilizers
10.4 11.5 4.2
Cattle manu-
re, 40 t/ha 40.1 52.4 12.2
with Biohran
Without
fertilizers 20.5 29.3 11.9
Cattle manu-
re, 40 t/ha 40.5 53.5 15.1
LSD05 2.2 3.0 2.6
manure. In other terms the manure application
ensures non-specific soil bacterization. Under
these conditions, microorganisms introduced
with the biological preparation into the agro-
cenosis undergo strong competition that on a
greater extend reduces their effectiveness.
The analysis of nitrogen activity indica-
tors highlights the environmentally favoura-
bility of both manure and microbial prepara-
tion application, since nitrogen fixation does
not occur under the excess of mineral nitrogen
compounds, thus supporting the expediency of
fertilizers use [11].
At the same time, a high level of biological
denitrification upon the introduction of 40 t/ha
manure was observed (Table 2). Since the si-
multaneous high values of the nitrogen fixation
activity and N2O emissions in the ecological
niche contradict the logic regarding the condi-
tions of their flow, we believe that the introduc-
tion of manure in the soil forms microzones,
where certain processes are dominated, includ-
ing the opposite ones. The obtained results had
also proved the need the classical approach to
manure preparation prior to its use through the
preliminary composting, which allows stabiliza-
tion of the population of microorganism in the
substrate and completion of the nitrogen and
carbon transformation processes.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
20
Table 2. The effect of organic fertilizer and
bacterization on the potential N2O emission of
rhizosphere soil of potato plants, 2015
Variants of
experiment
nmol N2O / g of dry soil per 24 h
Start of
budding
stage
Flower-
ing stage
Top
necrosis
stage
without bacterization
Control,
without
fertilizers
11.5 6.9 4.2
Cattle manu-
re, 40 t/ha 40.1 29.0 12.2
with Biohran
Without
fertilizers 10.0 6.3 3.5
Cattle manu-
re, 40 t/ha 40.5 30.5 13.1
LSD05 3.3 2.6 2.1
As is indicted in Table 2 the use of Biogran
on the background of manure application had
reduced the intensity of nitrous oxide emissions.
Determination of the orientation of nitrogen
fixation process in the root zone of barley plants
(next crop in the crop rotation), had revealed the
increase of nitrogen fixation activity on the ma-
nure after-effect background (Table 3).
The use of Microhumin for pre-seeding
bacterization of barley had also promoted the
significant stimulation of nitrogen fixation ac-
tivity. In contrast to the conditions prevailing in
the first year of manure use, the after-effect of
this organic fertilizer created a perfect back-
ground for the use of a microbial preparation in
crop growing technology, as the conditions de-
veloped during the first year of manure after-
effect in soil are very favourable for the effec-
tive microbial preparation performance.
This conclusion is confirmed by the deter-
mination of the potential emission of nitrous ox-
ide from rhizosphere soil of barley plants. In
particular, the use of Microhumin had reduced
the activity of biological denitrification both in
control and on organic fertilizers background
(Table 4). The reduction of gaseous losses of
nitrogen is most likely due to a decrease in the
content of nitrates in the soil caused by plant
bacterization. Bacterized plants require a greater
number of mineral nitrogen compounds, includ-
Table 3. The potential nitrogenase activity
of rhizosphere soil of barley plants under the
first year of manure after-effect and applica-
tion of Microhumin, 2015
Variants of
experiment
Nitrogenase activity,
nmol С2Н4 / g of dry soil per hour
Tillering
stage
Booting
stage
Milky-
wax ripe-
ness
without bacterization
Control,
without
fertilizers
2.0 1.9 3.0
After effect
of cattle ma-
nure, 40 t/ha
2.7 3.9 5.8
with Microhumin
Without
fertilizers 4.3 3.5 3.6
After-effect
of cattle ma-
nure, 40 t/ha
7.5 4.4 7.4
LSD05 0.5 0.5 0.8
Table 4. The potential emissions of N2O
from rhizosphere soil of barley plants under
the first year of after-effect of manure and use
of Microhumin, 2015
Variants of
experiment
nmol N2O / g of dry soil per 24 h
Tillering
stage
Booting
stage
Milky-
wax ripe-
ness
without bacterization
Control,
without
fertilizers
6.4 5.3 5.8
After effect
of cattle ma-
nure, 40 t/ha
13.0 12.1 12.0
with Microhumin
Without
fertilizers 4.2 3.6 5.3
After-effect
of cattle ma-
nure, 40 t/ha
10.8 10.3 11.7
LSD05 1.2 1.0 1.2
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
21
ing nitrates, which are the substrate basis for the
biological denitrification process to provide a
constructive metabolism. As a reflection of this,
we observe changes in the intensity of N2O
emission.
Determination of the symbiotic nitrogen
fixation activity in pea agrocenoses had shown
the process intensification under the use of Rhi-
zohumin (Table 5). The after-effect of manure
had a positive effect on the nitrogen fixation ac-
tivity, while introduction of the biological pre-
paration had resulted in minor changes of the
studied parameters.
Table 5. The symbiotic nitrogen fixation
activity in pea agrocenoses under the after-
effect of manure and application of Rhizo-
humin, 2015
Variants of
experiment
Nitrogenase activity,
nmol С2Н4 / plant per hour
Booting
stage
Budding
stage
Pod for-
mation
stage
without bacterization
Control,
without
fertilizers
7.1 10.8 10.6
After effect
of cattle ma-
nure, 40 t/ha
11.3 16.8 13.4
bacterization with Rhizohumin
Without
fertilizers 13.2 13.1 12.8
After-effect
of cattle ma-
nure, 40 t/ha
12.1 16.2 13.8
LSD05 2.4 2.2 1.6
At the same time, Rhizohumin application
had positively changed the process of biological
denitrification in the rhizosphere soil of pea
plants (Table 6), ensuring the reduction of N2O
emission on both studied backgrounds.
An overall low level of the potential nitro-
gen fixation activity was observed in the rhizo-
sphere soil of winter wheat plants while the af-
ter-effect of manure in general had ensured the
growth of studied parameters (Table 7). Micro-
bial preparation Polymyxobacterin had also in-
fluenced to some extent on the process inten-
Table 6. The potential emissions of N2O
from rhizosphere soil of pea plants under the
second year of after-effect of manure and
application of Rhizohumin, 2015
Variants of
experiment
nmol N2O / g of dry soil per 24 h
Booting
stage
Budding
stage
Pod for-
mation
stage
without bacterization
Control,
without
fertilizers
11.1 8.8 14.3
After effect
of cattle ma-
nure, 40 t/ha
17.9 20.6 30.4
with Rhizohumin
Without
fertilizers 7.1 7.0 10.9
After-effect
of cattle ma-
nure, 40 t/ha
14.0 20.5 26.9
LSD05 1.9 1.4 2.3
Table 7. The effect of bacterization and af-
ter-effect of organic fertilizer on the potential
nitrogen fixation activity of rhizosphere soil of
winter wheat plants, 2015
Variants of
experiment
nmol C2H4 / g of dry soil per hour
Tillering
stage
Booting
stage
Milky-
wax ripe-
ness
without bacterization
Control,
without
fertilizers
0.2 2.2 4.5
After effect
of cattle ma-
nure (third
year), 40 t/ha
0.4 2.7 5.6
bacterization with Polymyxobacterin
Without
fertilizers
0.2 2.3 6.0
After-effect
of cattle ma-
nure (third
year), 40 t/ha
0.2 2.2 7.1
LSD05 0.04 0.04 0.6
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
22
sity (not in direct way but through the changes
in plants development, as it is based on phos-
phate-mobilizing bacteria Paenibacillus po-
lymyxa KB, known as a producer of phytohor-
mones).
The gaseous nitrogen losses under the third
year of manure after-effect were significantly
higher comparing to the control values (Tab-
le 8), especially during the tillering stage of
wheta growth. However, Polymyxobacterin re-
duces biological denitrification activity in the
root zone of plants, which is probably due to the
intensification of the growth and development
of bacterized plants and the greater absorption
of mineral nitrogen compounds by plants.
Table 8. The effect of fertilizers and bacte-
rization on the potential biological denitrifi-
cation activity of rhizosphere soil of winter
wheat, 2015
Variants of
experiment
nmol N2O/g of soil per hour
Tillering
stage
Booting
stage
Milky-
wax ripe-
ness
without bacterization
Control,
without
fertilizers
9.7 7.5 5.4
After effect
of cattle ma-
nure (third
year), 40 t/ha
18.1 11.1 5.4
bacterization with Polymyxobacterin
Without
fertilizers
7.2 6.9 4.0
After-effect
of cattle ma-
nure (third
year), 40 t/ha
13.7 9.7 4.7
LSD05 1.0 1.5 0.8
Given peculiarities of the studied processes
were observed during all research years.
Analysis of crop yields in crop rotation had
indicated a high fertilization potential of cattle
manure (Table 9). The direct effect of manure
on potato yields provided an average of 73 % by
all studied parameters. Application of Biogran
upon the control background had ensure addi-
tional 9 % oy crop production, while was not
Table 9. Crop yields upon the application
of manure and biological preparations (avera-
ge over 5 years), t/ha
Variants of
experiment
Without
bacterization
With
bacterization
Potato
Without
fertilizers 15.6 17.0
Cattle manure,
40 t/ha 27.1 27.8
LSD05 1.2
Spring barley
Without
fertilizers 2.4 2.6
Cattle manure,
40 t/ha (after-
effect of the
first year)
2.9 3.3
LSD05 0.2
Pea
Without
fertilizers 2.2 2.5
Cattle manure,
40 t/ha (after-
effect of the
second year)
2.5 2.8
LSD05 0.2
Winter wheat
Without fertiliz-
ers 4.9 5.5
Cattle manure,
40 t/ha (after-
effect of the
third year)
6.2 6.5
LSD05 0.3
effective upon its use on the manure back-
ground. Thus, indicating no need in the use of
biological preparations during the same year of
manure introduction.
The after-effect of manure on the first year
had improved barley yield by providing extra
0.5 t/ha of grain. The interaction of the organic
background with Microhumin was proved to be
effective, ensuring the highest yield of 3.3 t/ha.
Additional 0.3 t/ha of peas were obtained
upon the second-year after-effect of cattle ma-
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
23
nure. The same figures were obtained also in
variants with Rhizohumin, providing extra
0.6 t/ha of peas upon the use of microbial prepa-
ration on the organic background.
The peas are known to be one of the best
pre-crops for winter wheat. In our experiment,
the cultivation of wheat after pea had yielded
4.9 t/ha of grain. The third-year after-effect of
manure had contributed to obtaining additional
1.3 t/ha. The application of the microbial prepa-
ration had increased the yield by 0.6 t/ha com-
paring to the control background and by 1.6 t/ha
comparing to the manure application (Table 9).
In order to ensure the objectivity of the
conclusions, we have transformed the yield in
the grain units since the different crops were
cultivated in the studied crop rotation, whose
products are difficult to compare.
The calculations shown in table 10 indicate
that the organic fertilizers (cattle manure,
40 t/ha) had provided an increase in grain units
in the range of 1.3 t/ha per crop rotation area.
Application of the biological preparations capa-
ble of optimization of the biological processes
course in agrocenoses had ensured extra 0.4 t/ha
of grain units. The combination of biological
preparations with organic background had pro-
vided additional 0.3 tons of grain units per hec-
tare of crop rotation area.
Thus, the study performed had shown that
the use of 40 t/ha of cattle manure ensures a
significant increase of the nitrogen fixation ac-
tivity in the root zone of plants. At the same
time, the introduction manure is accompanied
with the significant losses of gaseous nitrogen
compounds. The use of microbial preparations
may meaningfully optimize the course of bio-
logical nitrogen transformation processes in ag-
rocenoses. At the same time, in the year of ma-
nure introduction, the effectiveness of pre-
seeding bacterization is levelled, which should
be taken into the account when designing a fer-
tilization system in the organic production sys-
tem.
1. Томашевська О. А. Органічне виробниц-
тво в світі: реалії та перспективи / О. А. Тома-
шевська // Інноваційна економіка. — 2013. —
№ 6. — С. 161–164.
2. Антонец С. Поле без плуга — по велению
души / С. Антонец; записал П. Коротич // Наде-
жда планеты. — 2009. — № 6. — С. 8–11.
3. Писаренко В. М. Основні напрями інтег-
рованого захисту рослин в умовах органічного
землеробства / В. М. Писаренко // Вісник Полта-
вської державної аграрної академії. — 2008. —
№ 4. — С. 14−18.
4. Шикула М. К. Вплив мінімального обро-
бітку на родючість чорнозему / М. К. Шикула,
О. В. Демиденко // Вісник аграрної науки. —
2004. — № 8. — С. 18–23.
5. Докучаев В. В. К вопросу об открытии
при русских университетах кафедр почвоведения
и учение о микроорганизмах / В. В. Докучаев //
Избранные сочинения. — М. : Гос. издательство
с.-х. литературы, 1948. — Т. 2. — С. 290–318.
6. Мікробні препарати у землеробстві. Тео-
рія і практика : монографія / [Волкогон В. В.,
Надкернична О. В., Ковалевська Т. М. та ін.] ;
за ред. В. В. Волкогона. — К. : Аграрна наука,
2006. — 312 с.
7. Умаров М. М. Ацетиленовый метод изу-
чения азотфиксации в почвенно-микробиологи-
ческих исследованиях / М. М. Умаров // Почво-
ведение. — 1976. — № 11. — С. 119–123.
Table 10. The effect of organic fertilizer and biological preparations on the yield of
grain units
Variants of
experiment
Grain units by crops of crop rotation
(average for one of five years), t/ha
Grain units (average for
one of five years),
t/ha of crop rotation area
potato barley pea wheat
without bacterization
Without fertilizer 3.9 3.0 2.6 5.2 3.7
40 t/ha of manure 6.8 3.6 3.0 6.6 5.0
with bacterization
Without fertilizer 4.3 3.3 3.0 5.6 4.1
40 t/ha of manure 6.9 4.1 3.4 6.8 5.3
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
24
8. Методы почвенной микробиологии и био-
химии / [И. В. Асеева, И. П. Бабьева, Б. А. Бызов
и др.] ; под ред. Д. Г. Звягинцева. — М. : МГУ,
1991. — 304 с.
9. Експериментальна ґрунтова мікробіологія /
[В. В. Волкогон, О. В. Надкернична, Л. М. Ток-
макова та ін.]; за ред. В. В. Волкогона. — К. :
Аграрна наука, 2010. — 464 с.
10. Доспехов Б. А. Методика полевого опыта
с основами статистической обработки результатов
исследований / Б. А. Доспехов — [5-е изд.]. —
М. : Агропромиздат, 1985. — 351 с.
11. Визначення фізіологічно (екологічно)
доцільних доз мінерального азоту в технологіях
вирощування сільськогосподарських культур
(науково-методичні рекомендації) / [І. В. Гриник,
А. С. Заришняк, В. В. Волкогон та ін.]. — К.,
2010. — 35 с.
СПРЯМОВАНІСТЬ ПРОЦЕСІВ БІОЛО-
ГІЧНОЇ ТРАНСФОРМАЦІЇ
АЗОТУ В ҐРУНТІ ЗА ОРГАНІЧНОГО
ВИРОБНИЦТВА СІЛЬСЬКОГОСПО-
ДАРСЬКОЇ ПРОДУКЦІЇ
В. В. Волкогон, А. М. Москаленко,
С. Б. Дімова, М. А. Журба,
К. І. Волкогон, Л. М. Токмакова,
Н. П. Штанько, Н. В. Луценко
Інститут сільськогосподарської мікробіології
та агропромислового виробництва НААН,
м. Чернігів
У польовому стаціонарному досліді на
чорноземі вилуженому визначали вплив пря-
мої дії і післядії 40 т/га гною ВРХ на спрямо-
ваність процесів азотфіксації та біологічної
денітрифікації в кореневій зоні рослин кар-
топлі, ячменю ярого, гороху і пшениці озимої
за їх вирощування в сівозміні. Застосування
гною сприяє суттєвому зростанню актив-
ності азотфіксації і водночас забезпечує
високий рівень емісії N2O. Використання в
технологіях вирощування культур мікробних
препаратів для передпосівної бактеризації
насіннєвого матеріалу оптимізує перебіг
процесів біологічної трансформації азоту —
зростає активність азотфіксації і зменшу-
ються газоподібні втрати елементу. Виня-
тком є застосування препарату Біограну
для картоплі в рік внесення гною. Надход-
ження до ґрунту великої кількості мікро-
організмів з гноєм нівелює позитивний
ефект біопрепарату. Облік урожаю та роз-
рахунок виходу зернових одиниць з гектара
сівозмінної площі свідчить про доцільність
поєднання гною та мікробних препаратів (за
виключенням року прямої дії органічного до-
брива) у системі органічного виробництва.
Ключові слова: органічне виробництво
сільськогосподарської продукції, мікробні
препарати, азотфіксація, емісія N2O, кар-
топля, ячмінь, горох, пшениця.
НАПРАВЛЕННОСТЬ ПРОЦЕССОВ
БИОЛОГИЧЕСКОЙ ТРАНСФОРМА-
ЦИИ АЗОТА В ПОЧВЕ ПРИ ОРГАНИ-
ЧЕСКОМ ПРОИЗВОДСТВЕ СЕЛЬСКО-
ХОЗЯЙСТВЕННОЙ ПРОДУКЦИИ
В. В. Волкогон, А. М. Москаленко,
С. Б. Димова, М. А. Журба,
Е. И. Волкогон, Л. Н. Токмакова,
Н. П. Штанько, Н. В. Луценко
Институт сельскохозяйственной микробиологии
и агропромышленного производства НААН,
г. Чернигов
В полевом стационарном опыте на черно-
зёме выщелоченном изучали влияние прямого
действия и последействия навоза ВРХ на на-
правленность процессов азотфиксации и био-
логической денитрификации в корневой зоне
растений картофеля, ячменя ярового, гороха и
пшеницы озимой при их выращивании в сево-
обороте. Применение навоза способствует
существенному возрастанию активности
азотфиксации и в то же время обеспечивает
высокий уровень эмиссии N2O. Использование
в технологиях выращивания культур микроб-
ных препаратов для предпосевной обработки
посевного материала оптимизирует направ-
ленность процессов биологической трансфор-
мации азота — возрастает активность
азотфиксации и уменьшаются газообразные
потери элемента. Исключением является
применение препарата Биограна для карто-
феля в год внесения навоза. Поступление в
почву вместе с навозом огромного количества
микроорганизмов нивелирует положительный
эффект биопрепарата. Учёт урожая и рас-
чёт выхода зерновых единиц с гектара сево-
оборотной площади свидетельствует о целе-
сообразности сочетания навоза и микробных
препаратов (исключением является год пря-
мого действия органического удобрения) в
системе органического производства.
Ключевые слова: органическое производ-
ство сельскохозяйственной продукции, мик-
робные препараты, азотфиксация, эмиссия
N2O, картофель, ячмень, горох, пшеница.
ISSN 1997-3004 Сільськогосподарська мікробіологія. — 2017. — Вип. 25.
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| id | oai:ojs2.smic.in.ua:article-86 |
| institution | Agriciltural microbiology |
| keywords_txt_mv | keywords |
| language | English |
| last_indexed | 2026-07-23T01:03:42Z |
| publishDate | 2017 |
| publisher | Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine |
| record_format | ojs |
| resource_txt_mv | smicinua/f0/d9444073abcb32d7a6c12cd5681e23f0.pdf |
| spelling | oai:ojs2.smic.in.ua:article-862026-07-22T10:10:41Z THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS Volkohon, V. V. Moskalenko, A. M. Dimova, S. B. Zhurba, M. A. Volkohon, K. I. Tokmakova, L. M. Shtanko, N. P. Lutsenko, N. V. organic agriculture, microbial preparations, nitrogen fixation, N2O emission, potato, barley, pea, wheat. organic agriculture, microbial preparations, nitrogen fixation, N2O emission, potato, barley, pea, wheat. The paper covers the study of direct impact and after-effect of 40 t/ha of cattle manure on theorientation of nitrogen fixation and biological denitrification processes in the root zone of potatoes,spring barley, pea, and winter wheat plants in rotation in a stationary field experiments on leachedblack soil. Application of manure had significantly increased the nitrogen fixation activity, whilepromoting a high level of N2O emission. The use of microbial preparations for pre-seeding bacterization of seeds optimizes the course of biological nitrogen transformation process — through theenhancement of nitrogen fixation activity and reduction of gaseous nitrogen losses (with the exception of Biogran use on potatoes in the year of manure application). Introduction with manure of alarge number of microorganisms to the soil offsets the positive effect of biopreparations use. Yieldrecords and estimation of grain output per hectare within the crop rotation cycle indicates the practicability of combined application of manure and microbial preparations (excluding the year of direct effect of organic fertilizer) in organic agriculture. The paper covers the study of direct impact and after-effect of 40 t/ha of cattle manure on theorientation of nitrogen fixation and biological denitrification processes in the root zone of potatoes,spring barley, pea, and winter wheat plants in rotation in a stationary field experiments on leachedblack soil. Application of manure had significantly increased the nitrogen fixation activity, whilepromoting a high level of N2O emission. The use of microbial preparations for pre-seeding bacterization of seeds optimizes the course of biological nitrogen transformation process — through theenhancement of nitrogen fixation activity and reduction of gaseous nitrogen losses (with the exception of Biogran use on potatoes in the year of manure application). Introduction with manure of alarge number of microorganisms to the soil offsets the positive effect of biopreparations use. Yieldrecords and estimation of grain output per hectare within the crop rotation cycle indicates the practicability of combined application of manure and microbial preparations (excluding the year of direct effect of organic fertilizer) in organic agriculture. Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2017-06-29 Article Article Рецензована Стаття application/pdf https://smic.in.ua/index.php/journal/article/view/86 10.35868/1997-3004.25.18-24 Agricultural microbiology; Vol. 25 (2017): Agriciltural microbiology; 18-24 Сільськогосподарська мікробіологія; Том 25 (2017): Сільськогосподарська мікробіологія; 18-24 1997-3004 10.35868/1997-3004.25 en https://smic.in.ua/index.php/journal/article/view/86/74 Авторське право (c) 2017 V. V. Volkohon, A. M. Moskalenko, S. B. Dimova, M. A. Zhurba, K. I. Volkohon, L. M. Tokmakova, N. P. Shtanko, N. V. Lutsenko https://creativecommons.org/licenses/by/4.0 |
| spellingShingle | organic agriculture microbial preparations nitrogen fixation N2O emission potato barley pea wheat. Volkohon, V. V. Moskalenko, A. M. Dimova, S. B. Zhurba, M. A. Volkohon, K. I. Tokmakova, L. M. Shtanko, N. P. Lutsenko, N. V. THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_alt | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_full | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_fullStr | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_full_unstemmed | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_short | THE ORIENTATION OF BIOLOGICAL NITROGEN TRANSFORMATION PROCESSES IN SOIL UNDER THE ORGANIC PRODUCTION OF AGRICULTURAL PRODUCTS |
| title_sort | orientation of biological nitrogen transformation processes in soil under the organic production of agricultural products |
| topic | organic agriculture microbial preparations nitrogen fixation N2O emission potato barley pea wheat. |
| topic_facet | organic agriculture microbial preparations nitrogen fixation N2O emission potato barley pea wheat. organic agriculture microbial preparations nitrogen fixation N2O emission potato barley pea wheat. |
| url | https://smic.in.ua/index.php/journal/article/view/86 |
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