СПРЯМОВАНІСТЬ МІКРОБІОЛОГІЧНИХ ПРОЦЕСІВ У КОНСЕРВОВАНОМУ ПЛЮЩЕНОМУ ЗЕРНІ КУКУРУДЗИ ЗА ВПЛИВУ БАКТЕРІЙ BACILLUS SUBTILIS

• Дата: 2018
• Автори: Передерій, М.Г., Кравченко, Н.О., Божок, Л.В., Дмитрук, О.М.
• Формат: Стаття
• Мова: English
• Опубліковано: Institute of Agrocultural Microbiology and Agro-industrial Manufacture of NAAS of Ukraine 2018
• Теми: Bacillus subtilis; corn rolled grain; biological preservatives; yeasts; moulds; preservation; плющене зерно кукурудзи; біологічні консерванти; дріжджі; плісеневі гриби; консервування
• Онлайн доступ: https://smic.in.ua/index.php/journal/article/view/9
• Назва журналу: Agriciltural microbiology

Objective. To study the trends of microbiological processes, changes in the level of acidity and the ratio of accumulated organic acids in preserved corn rolled grain due to the influence of selected strains of Bacillus subtilis, promising for the creation of biological preservatives on their basis. Methods. Microbiological, zootechnical. Results. Grain processing with suspensions of the test strains of B. subtilis promotes the intensive development of epiphytic lactic acid bacteria in feed at day 15 of preservation, with subsequent gradual decrease at day 30 and 70 of the experiment. The number of lactic acid bacteria at day 14 after opening samples of preserved corn rolled grain is a tenfold higher than in the control variant (without the additional application of microorganisms). The dynamics of yeast growth in the feed due to the effect of the test strains of B. subtilis is characterized by their intensive development at day 15 with a gradual decrease in the number in all variants at day 30 and 70 of preservation. The number of moulds in the control variant exceeds the maximum allowable level (up to 5×104 CFU per 1 g feed) and is 2×105 at day 7 and 6.2×105 at day 14 after opening and access of air to the thickness of the preserved mass. In experimental variants, the number of moulds is smaller compared with the control both in the process of preservation and after opening the feed. The optimum pH and the ratio of organic acids at day 14 after opening has been registered in the rolled grain treated with B. subtilis B1, B. subtilis B6y and B. subtilis 44-p. Conclusion. The use of selected B. subtilis strains to preserve moist corn rolled grain promotes the establishment of the optimum level of acidity of the feed and the ratio of organic acids, inhibition of the development of undesirable microorganisms.