Влияние сахаров на дегидратацию биополимерных молекул в процессе криоконсервирования

Влияние сахаров на дегидратацию биополимерных молекул в процессе криоконсервирования

Protein molecules’ hydration in the presence of sugars: glucose, fructose and sucrose were studied by NMR-spectroscopy. The parameters of hydration, concentration of bound water and value of interfacial energy were determined over a wide range of component concentrations. It was shown with exempli...

Full description

Saved in:
Bibliographic Details
Date:2007
Main Authors: Rugal, A. A., Barvinchenko, V. N., Galagan, N. P., Siora, I. V., Turov, V. V.
Format: Article
Language:English
Published: Publishing House ‘Akademperiodyka’ of the National Academy of Sciences of Ukraine; Institute for Problems of Cryobiology and Cryomedicine 2007
Subjects:
кріоконсервування
кріопротектори
ЯМР-спектроскопія
гідратація
зв’язана вода
міжфазна енергія
Online Access:https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/484
Tags: Add Tag
No Tags, Be the first to tag this record!
Journal Title:Problems of Cryobiology and Cryomedicine

Institution

Problems of Cryobiology and Cryomedicine
Description
Summary:Protein molecules’ hydration in the presence of sugars: glucose, fructose and sucrose were studied by NMR-spectroscopy. The parameters of hydration, concentration of bound water and value of interfacial energy were determined over a wide range of component concentrations. It was shown with exemplifying using the serum albumin, that low-molecular compounds (sugars) could replace an intraglobular water. The maximal effect of dehydration was observed for sucrose. In this case protein molecules change their property and capability to be adsorbed on a surface of fine-grained silica, which is reduced by 25% as a result of the forming complexes.

Similar Items